Department of Respiratory Medicine, Second Military Medical University/Nanjing General Hospital of Nanjing Military Command, People's Liberation Army, Nanjing, Jiangsu, China.
Chin Med J (Engl). 2012 Feb;125(4):617-21.
Aspergillus fumigatus (A. fumigatus) is a ubiquitous saprophytic fungus responsible for the majority of invasive mold infections in patients undergoing chemotherapy, organ transplantation or with persistent neutropenia. This study aimed to determine the role of E-cadherin for adhesion and endocytosis of A. fumigatus blastospores in the human epithelial cell line A549.
A. fumigatus blastospores were incubated with the total protein of A549 to investigate the binding of E-cadherin and blastospores followed by an affinity purification procedure. After establishing the adhesion model, the adhesion and endocytosis of A. fumigatus blastospores by A549 cells were evaluated by down-regulating E-cadherin of A549 cells using blocking antibody or small interfering RNA (siRNA).
E-cadherin was adhered to the surface of A. fumigatus blastospore. Adhesion and endocytosis of the blastospores were reduced by blocking or down-regulating E-cadherin in A549 cells.
E-cadherin is a receptor for adhesion and endocytosis of A. fumigatus blastospores in epithelial cells. This may open a new approach to treat this fungal infection.
烟曲霉（A. fumigatus）是一种无处不在的腐生真菌，负责引起接受化疗、器官移植或持续中性粒细胞减少的患者中大多数侵袭性霉菌感染。本研究旨在确定 E-钙黏蛋白在人上皮细胞系 A549 中对烟曲霉芽生孢子的黏附和内吞作用中的作用。
用 A549 的总蛋白孵育烟曲霉芽生孢子，以研究 E-钙黏蛋白与芽生孢子的结合，然后进行亲和纯化程序。在建立黏附模型后，使用阻断抗体或小干扰 RNA（siRNA）下调 A549 细胞中的 E-钙黏蛋白，评估 A549 细胞中烟曲霉芽生孢子的黏附和内吞作用。
E-钙黏蛋白附着在烟曲霉芽生孢子的表面。在 A549 细胞中阻断或下调 E-钙黏蛋白会降低芽生孢子的黏附和内吞作用。
E-钙黏蛋白是上皮细胞中烟曲霉芽生孢子黏附和内吞的受体。这可能为治疗这种真菌感染开辟了新途径。
