Max-Bergmann-Center for Biomaterials, Technische Universität Dresden, Dresden, Germany.
Eur Cell Mater. 2012 Apr 10;23:237-47; discussion 247-8. doi: 10.22203/ecm.v023a18.
Histological imaging is still considered the gold standard for analysing bone formation around metallic implants. Generally, a limited number of histological sections per sample are used for the approximation of mean values of peri-implant bone formation. In this study we compared statistically the results of bone-implant contact (BIC) and bone-implant volume (BIV) obtained by histological sections, with those obtained by X-ray absorption images from synchrotron radiation micro-computed tomography (SRµCT) using osseointegrated screw-shaped implants from a mini-pig study. Comparing the BIC results of 3-4 histological sections per implant sample with the appropriate 3-4 SRµCT slices showed a non-significant difference of 1.9 % (p = 0.703). The contact area assessed by the whole 3D information from the SRµCT measurement in comparison to the histomorphometric results showed a non-significant difference in BIC of 4.9 % (p = 0.171). The amount of the bone-implant volume in the histological sections and the appropriate SRµCT slices showed a non-significant difference by only 1.4 % (p = 0.736) and also remains non-significant with 2.6 % (p = 0.323) using the volumetric SRµCT information. We conclude that for a clinical evaluation of implant osseointegration with histological imaging at least 3-4 sections per sample are sufficient to represent the BIC or BIV for a sample. Due to the fact that in this study we have found a significant intra-sample variation in BIC of up to ± 35 % the selection of only one or two histological sections per sample may strongly influence the determined BIC.
组织学成像仍然被认为是分析金属植入物周围骨形成的金标准。通常，每个样本使用有限数量的组织学切片来近似种植体周围骨形成的平均值。在这项研究中，我们比较了统计学上通过组织学切片获得的骨-种植体接触（BIC）和骨-种植体体积（BIV）的结果，以及使用来自迷你猪研究的骨整合螺钉形植入物的同步辐射微计算机断层扫描（SRµCT）的 X 射线吸收图像获得的结果。将每个植入物样本的 3-4 个组织学切片的 BIC 结果与适当的 3-4 个 SRµCT 切片进行比较，结果显示差异无统计学意义，为 1.9%（p=0.703）。与组织形态计量学结果相比，通过 SRµCT 测量的整个 3D 信息评估的接触面积显示 BIC 的差异无统计学意义，为 4.9%（p=0.171）。组织学切片和适当的 SRµCT 切片中的骨-种植体体积差异无统计学意义，仅为 1.4%（p=0.736），使用体积 SRµCT 信息也无统计学意义，为 2.6%（p=0.323）。我们得出结论，对于植入物骨整合的临床评估，每个样本至少需要 3-4 个切片进行组织学成像，以代表样本的 BIC 或 BIV。由于在这项研究中，我们发现 BIC 的样本内变异高达±35%，因此每个样本仅选择一个或两个组织学切片可能会强烈影响确定的 BIC。
