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用人口腔微生物群中筛选出的菌株诱导实验性种植体周围炎

Induction of Experimental Peri-Implantitis with Strains Selected from the Human Oral Microbiome.

作者信息

Ancuţa Diana Larisa, Alexandru Diana Mihaela, Crivineanu Maria, Coman Cristin

机构信息

Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine, 050097 Bucharest, Romania.

Cantacuzino National Medical Military Institute for Research and Development, 050096 Bucharest, Romania.

出版信息

Biomedicines. 2024 Mar 22;12(4):715. doi: 10.3390/biomedicines12040715.

DOI:10.3390/biomedicines12040715
PMID:38672071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11048198/
Abstract

Peri-implantitis (PI), the most widespread condition in the oral cavity, affects patients globally; thus, advanced research in both in vitro and in vivo studies is required. This study aimed to develop peri-implantitis in the rat model by oral contamination with bacteria responsible for PI in humans. The study was carried out in three stages: the extraction of the maxillary first molar to reproduce the human edentation, the mounting of the implant, and finally, the contamination of the device by gavage with , and . The hematological examinations showed statistically significant increases for WBCs (white blood cells), Hb (hemoglobin), RBCs (red blood cells), MCH (mean corpuscular hemoglobin), MCHC (mean corpuscular hemoglobin concentration), and PLTs (platelets), but especially for the level of neutrophils and lymphocytes, and the systemic immunoinflammatory index completed the picture related to the inflammatory response triggered as a result of the activity of microorganisms pathogens on oral tissues. By examining the liver and kidney profile, we hypothesized that peri-implantitis is associated with systemic diseases, and the histopathological examination showed peri-implantitis lesions characterized by a marked inflammatory infiltrate with numerous neutrophils and lymphocytes. By corroborating all the results, we successfully developed a rat peri-implantitis model using a mixed bacterial infection through the oral gavage technique.

摘要

种植体周围炎（PI）是口腔中最普遍的病症，全球患者均受其影响；因此，需要在体外和体内研究方面开展深入研究。本研究旨在通过用导致人类PI的细菌经口污染，在大鼠模型中诱发种植体周围炎。该研究分三个阶段进行：拔除上颌第一磨牙以重现人类无牙状态、植入种植体，最后通过灌胃用 、 和 对种植体进行污染。血液学检查显示，白细胞（WBC）、血红蛋白（Hb）、红细胞（RBC）、平均红细胞血红蛋白含量（MCH）、平均红细胞血红蛋白浓度（MCHC）和血小板（PLT）有统计学意义的升高，尤其是中性粒细胞和淋巴细胞水平，全身免疫炎症指数完善了与微生物病原体对口腔组织的作用引发的炎症反应相关的情况。通过检查肝肾功能，我们推测种植体周围炎与全身性疾病有关，组织病理学检查显示种植体周围炎病变的特征是有大量中性粒细胞和淋巴细胞的明显炎症浸润。通过综合所有结果，我们通过经口灌胃技术使用混合细菌感染成功建立了大鼠种植体周围炎模型。

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