Department of Oral and Maxillofacial Surgery, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.
Department of Oral and Maxillofacial Surgery, Ludwig-Maximilians-University of Munich, Munich, Germany.
J Clin Periodontol. 2017 Apr;44(4):418-427. doi: 10.1111/jcpe.12693. Epub 2017 Feb 27.
The bone implant contact (BIC) has traditionally been evaluated with histological methods. Thereupon, strong correlations of two-dimensional (2D) BIC have been detected between μCT and destructive histology. However, due to the high intra-sample variability in BIC values, one histological slice is not sufficient to represent 3D BIC. Therefore, our aim has been to correlate the averaged values of 3-4 histological sections to 3D μCT.
Fifty-four implants inserted into the maxilla of 14 minipigs were evaluated. Two different time points were selected to assess the 3D BIC (distance to implant: 2-5 voxels), an inner ring (6-30 voxels) and an outer ring (55-100 voxels) using μCT (voxel size: 10 μm) and to correlate the values to histomorphometry.
Strong correlations (p < 0.0001; 28 days, 56 days, total) were seen between μCT and histomorphometry concerning BIC (r = 0.84, r = 0.85, r = 0.83), the inner ring (r = 0.87, r = 0.87, r = 0.88) and the outer ring (r = 0.85, r = 0.85, r = 0.88). Closer to the implant, μCT values were higher compared with histomorphometry.
Although 3-4 histological slices per implant seem to predict the 3D BIC, μCT might be advantageous because of its non-destructive 3D character. The healing time may not impact on the comparability.
骨植入物接触（BIC）传统上通过组织学方法进行评估。因此，在μCT 和破坏性组织学之间检测到二维（2D）BIC 的强相关性。然而，由于 BIC 值的样本内变异性较大，一个组织切片不足以代表 3D BIC。因此，我们的目标是将 3-4 个组织切片的平均值与 3D μCT 相关联。
评估了 14 只小型猪上颌骨中插入的 54 个植入物。选择两个不同的时间点使用μCT（体素大小：10μm）评估 3D BIC（植入物距离：2-5 体素）、内圈（6-30 体素）和外圈（55-100 体素），并将值与组织形态计量学相关联。
在 28 天和 56 天以及总时间点，μCT 和组织形态计量学之间的 BIC（r=0.84、r=0.85、r=0.83）、内圈（r=0.87、r=0.87、r=0.88）和外圈（r=0.85、r=0.85、r=0.88）之间观察到强烈的相关性（p<0.0001）。与植入物更接近的位置，μCT 值高于组织形态计量学。
尽管每个植入物似乎需要 3-4 个组织切片来预测 3D BIC，但μCT 可能具有优势，因为它具有非破坏性的 3D 特性。愈合时间可能不会影响可比性。
