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FEMS Microbiol Ecol. 2011 Nov;78(2):220-32. doi: 10.1111/j.1574-6941.2011.01146.x. Epub 2011 Jul 4.
2
cis-antisense RNA, another level of gene regulation in bacteria.顺式反义 RNA,细菌中基因调控的另一层次。
Microbiol Mol Biol Rev. 2011 Jun;75(2):286-300. doi: 10.1128/MMBR.00032-10.
3
RNase III participates in GadY-dependent cleavage of the gadX-gadW mRNA.RNase III 参与 GadY 依赖型的 gadX-gadW mRNA 的切割。
J Mol Biol. 2011 Feb 11;406(1):29-43. doi: 10.1016/j.jmb.2010.12.009. Epub 2010 Dec 13.
4
Widespread antisense transcription in Escherichia coli.大肠杆菌中广泛的反义转录。
mBio. 2010 May 18;1(1):e00024-10. doi: 10.1128/mBio.00024-10.
5
Many chromosomal genes modulate MarA-mediated multidrug resistance in Escherichia coli.许多染色体基因调节大肠杆菌中 MarA 介导的多药耐药性。
Antimicrob Agents Chemother. 2010 May;54(5):2125-34. doi: 10.1128/AAC.01420-09. Epub 2010 Mar 8.
6
Transcriptome analysis of Pseudomonas syringae identifies new genes, noncoding RNAs, and antisense activity.丁香假单胞菌转录组分析鉴定新基因、非编码 RNA 和反义活性。
J Bacteriol. 2010 May;192(9):2359-72. doi: 10.1128/JB.01445-09. Epub 2010 Feb 26.
7
Requirement of polyphosphate by Pseudomonas fluorescens Pf0-1 for competitive fitness and heat tolerance in laboratory media and sterile soil.荧光假单胞菌Pf0-1在实验室培养基和无菌土壤中对多聚磷酸盐的需求,以实现竞争适应性和耐热性。
Appl Environ Microbiol. 2009 Jun;75(12):3872-81. doi: 10.1128/AEM.00017-09. Epub 2009 Apr 24.
8
PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria.基于PBAD的穿梭载体,用于对假单胞菌属、伯克霍尔德菌属及其他细菌中有毒且调控严格的基因进行功能分析。
Appl Environ Microbiol. 2008 Dec;74(23):7422-6. doi: 10.1128/AEM.01369-08. Epub 2008 Oct 10.
9
GadX/GadW-dependent regulation of the Escherichia coli acid fitness island: transcriptional control at the gadY-gadW divergent promoters and identification of four novel 42 bp GadX/GadW-specific binding sites.大肠杆菌酸适应性岛的GadX/GadW依赖性调控:gadY - gadW分歧启动子处的转录控制及四个新的42 bp GadX/GadW特异性结合位点的鉴定
Mol Microbiol. 2008 Nov;70(4):965-82. doi: 10.1111/j.1365-2958.2008.06458.x. Epub 2008 Sep 18.
10
Overlapping protein-encoding genes in Pseudomonas fluorescens Pf0-1.荧光假单胞菌Pf0-1中的重叠蛋白质编码基因。
PLoS Genet. 2008 Jun 13;4(6):e1000094. doi: 10.1371/journal.pgen.1000094.

荧光假单胞菌 Pf0-1 中反义 RNA 对多聚磷酸盐激酶合成的调控。

Regulation of polyphosphate kinase production by antisense RNA in Pseudomonas fluorescens Pf0-1.

机构信息

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.

出版信息

Appl Environ Microbiol. 2012 Jun;78(12):4533-7. doi: 10.1128/AEM.07836-11. Epub 2012 Apr 6.

DOI:10.1128/AEM.07836-11
PMID:22492458
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3370510/
Abstract

Pseudomonas spp. adapt rapidly to environmental fluctuations. Loss or overproduction of polyphosphate reduces the fitness of Pseudomonas fluorescens Pf0-1, indicating the importance of the fine-tuning of polyphosphate production. An antisense RNA was investigated and shown to regulate the polyphosphate kinase gene (ppk) by a posttranscriptional mechanism reducing ppk transcript abundance.

摘要

铜绿假单胞菌能够迅速适应环境波动。聚磷酸盐的缺失或过表达会降低荧光假单胞菌 Pf0-1 的适应性,表明聚磷酸盐产生的精细调控具有重要意义。本文研究了一种反义 RNA,发现它通过一种转录后机制调节聚磷酸激酶基因(ppk),降低 ppk 转录本的丰度。