荧光假单胞菌 Pf0-1 中反义 RNA 对多聚磷酸盐激酶合成的调控。
Regulation of polyphosphate kinase production by antisense RNA in Pseudomonas fluorescens Pf0-1.
机构信息
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.
出版信息
Appl Environ Microbiol. 2012 Jun;78(12):4533-7. doi: 10.1128/AEM.07836-11. Epub 2012 Apr 6.
Abstract
Pseudomonas spp. adapt rapidly to environmental fluctuations. Loss or overproduction of polyphosphate reduces the fitness of Pseudomonas fluorescens Pf0-1, indicating the importance of the fine-tuning of polyphosphate production. An antisense RNA was investigated and shown to regulate the polyphosphate kinase gene (ppk) by a posttranscriptional mechanism reducing ppk transcript abundance.
摘要
铜绿假单胞菌能够迅速适应环境波动。聚磷酸盐的缺失或过表达会降低荧光假单胞菌 Pf0-1 的适应性，表明聚磷酸盐产生的精细调控具有重要意义。本文研究了一种反义 RNA，发现它通过一种转录后机制调节聚磷酸激酶基因（ppk），降低 ppk 转录本的丰度。
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