Department of Virology, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands.
J Infect Dis. 2012 Jun 15;205(12):1877-84. doi: 10.1093/infdis/jis284. Epub 2012 Apr 5.
Seasonal hyperacute panuveitis (SHAPU) is a potentially blinding ocular disease occurring in Nepal that principally affects young children. Random amplification of partially purified vitreous fluid (VF)-derived nucleic acid revealed the presence of human anelloviruses in VF of SHAPU patients. In a comparative study of patients with different ocular pathologies, SHAPU patients were at highest risk of harboring anelloviruses in their eyes. The majority of SHAPU patients had multiple anelloviruses in their VF. The ocular anellovirus load in SHAPU and non-SHAPU patients did not differ and no SHAPU-specific anellovirus variant was detected. Analysis of paired serum and VF samples from SHAPU and non-SHAPU patients showed that the anellovirus detected in VF samples most likely originated from the systemic viral pool during viremia, potentially through breakdown of the blood-ocular barrier. The detection of anelloviruses in VF samples of uveitis patients, profoundly so in SHAPU patients, is imperative and warrants elucidation of its clinical significance.
季节性超急性全葡萄膜炎（SHAPU）是一种在尼泊尔发生的潜在致盲性眼病，主要影响幼儿。对部分纯化的玻璃体（VF）衍生核酸的随机扩增显示，SHAPU 患者的 VF 中存在人类细小病毒。在对不同眼部疾病患者的比较研究中，SHAPU 患者眼睛携带细小病毒的风险最高。大多数 SHAPU 患者的 VF 中存在多种细小病毒。SHAPU 和非 SHAPU 患者的眼部细小病毒载量没有差异，也没有检测到 SHAPU 特异性细小病毒变异体。对 SHAPU 和非 SHAPU 患者的配对血清和 VF 样本进行分析表明，在 VF 样本中检测到的细小病毒很可能来源于病毒血症期间的全身病毒库，可能是通过血眼屏障的破坏。细小病毒在葡萄膜炎患者的 VF 样本中的检测，尤其是在 SHAPU 患者中，至关重要，需要阐明其临床意义。
