Graduate School of Biomedical Engineering, University of New South Wales, Sydney 2052, Australia.
Macromol Biosci. 2012 Mar;12(3):322-32. doi: 10.1002/mabi.201100292.
Sericin peptides and PVA are chemically modified with methacrylate groups to produce a covalent PVA/sericin hydrogel. Preservation of the sericin bioactivity following methacrylation is confirmed, and PVA/sericin hydrogels are fabricated for both B. mori and A. mylitta sericin. Cell adhesion studies confirm the preservation of sericin bioactivity post incorporation in PVA gels. PVA/A. mylitta gels are observed to facilitate cell adhesion to a significantly greater degree than PVA/B. mori gels. Overall, the incorporation of sericin does not alter the physical properties of the PVA hydrogels but does result in significantly improved cellular interaction, particularly from A. mylitta gels.
丝胶肽和 PVA 用丙烯酰胺基团进行化学修饰，以生产共价 PVA/丝胶水凝胶。丝胶的生物活性在丙烯酰胺化后得到了保留，并为 B. mori 和 A. mylitta 丝胶制备了 PVA/丝胶水凝胶。细胞黏附研究证实，丝胶生物活性在掺入 PVA 凝胶后得到了保留。与 PVA/B. mori 凝胶相比，PVA/A. mylitta 凝胶显著促进了细胞黏附。总的来说，丝胶的掺入并没有改变 PVA 水凝胶的物理性质，但确实导致细胞相互作用得到了显著改善，特别是来自 A. mylitta 凝胶的改善。
