Suktham Kunat, Koobkokkruad Thongchai, Saesoo Somsak, Saengkrit Nattika, Surassmo Suvimol
Nano Delivery System Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Nano-Cosmeceutical Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Colloids Surf B Biointerfaces. 2016 Dec 1;148:487-495. doi: 10.1016/j.colsurfb.2016.09.024. Epub 2016 Sep 21.
Sericin protein (SP) is widely used as a nutrient biomaterial for biomedical and cosmeceutical applications although it shows low stability to heat and light. To overcome these problems and add value to wastewater from the silk industry, sericin protein was recovered as sericin-loaded copolymer-liposomes (SP-PVA-LP), prepared through thin film hydration. The size and morphology of the liposomes were investigated using dynamic light scattering (DLS), and electron microscopy (SEM and TEM). The particle size, liposome surface morphology and encapsulation efficiency of SP were dependent on PVA concentration. The hydrodynamic size of the nanoparticles was between 200 and 400nm, with the degree of negative charge contingent on sericin loading. SEM and TEM images confirmed the mono-dispersity, and spherical nature of the particles, with FTIR measurements confirming the presence of surface bound PVA. Exposure of liposomes to 500ppm sericin highlighted a dependence of encapsulation efficiency on PVA content; 2% surface PVA proved the optimal level for sericin loading. Cytotoxicity and viability assays revealed that SP-loaded surface modified liposomes promote cellular attachment and proliferation of human skin fibroblasts without adverse toxic effects. Surface modified copolymer liposomes show high performance in maintaining structural stability, and promoting enhancements in the solubility and bio-viability of sericin. Taken together, these biocompatible constructs allow for effective controlled release, augmenting sericin activity and resulting in effective drug delivery systems.
丝胶蛋白（SP）虽对热和光稳定性较低，但作为一种营养生物材料在生物医学和化妆品应用中被广泛使用。为克服这些问题并提升丝绸工业废水的价值，通过薄膜水化法制备了载有丝胶蛋白的共聚物脂质体（SP-PVA-LP）来回收丝胶蛋白。使用动态光散射（DLS）、电子显微镜（SEM和TEM）研究了脂质体的大小和形态。脂质体的粒径、表面形态和丝胶蛋白的包封率取决于聚乙烯醇（PVA）的浓度。纳米颗粒的流体动力学尺寸在200至400纳米之间，负电荷程度取决于丝胶蛋白的负载量。SEM和TEM图像证实了颗粒的单分散性和球形性质，傅里叶变换红外光谱（FTIR）测量证实了表面结合PVA的存在。将脂质体暴露于500ppm丝胶蛋白中，结果表明包封率取决于PVA含量；2%的表面PVA被证明是丝胶蛋白负载的最佳水平。细胞毒性和活力测定表明，载有丝胶蛋白的表面修饰脂质体可促进人皮肤成纤维细胞的细胞附着和增殖，且无不良毒性作用。表面修饰的共聚物脂质体在维持结构稳定性以及提高丝胶蛋白的溶解度和生物活性方面表现出高性能。综上所述，这些生物相容性构建体可实现有效的控释，增强丝胶蛋白活性，从而形成有效的药物递送系统。
