Han Chaoshan, Liu Feng, Zhang Yu, Chen Wenjie, Luo Wei, Ding Fengzhi, Lu Lin, Wu Chengjie, Li Yangxin
Department of Cardiovascular Surgery, Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, First Affiliated Hospital and Medical College of Soochow University, Suzhou, China.
Front Cardiovasc Med. 2021 Aug 19;8:713021. doi: 10.3389/fcvm.2021.713021. eCollection 2021.
Recent studies have shown that the hydrogels formed by composite biomaterials are better choice than hydrogels formed by single biomaterial for tissue repair. We explored the feasibility of the composite hydrogel formed by silk fibroin (SF) and silk sericin (SS) in tissue repair for the excellent mechanical properties of SF, and cell adhesion and biocompatible properties of SS. In our study, the SF SS hydrogel was formed by SF and SS protein with separate extraction method (LiBr dissolution for SF and hot alkaline water dissolution for SS), while SF-SS hydrogel was formed by SF and SS protein using simultaneous extraction method (LiBr dissolution for SF and SS protein). The effects of the two composite hydrogels on the release of inflammatory cytokines from macrophages and the wound were analyzed. Moreover, two hydrogels were used to encapsulate and deliver human umbilical cord mesenchymal stem cell derived exosomes (UMSC-Exo). Both SF SS and SF-SS hydrogels promoted wound healing, angiogenesis, and reduced inflammation and TNF-α secretion by macrophages. These beneficial effects were more significant in the experimental group treated by UMSC-Exo encapsulated in SF-SS hydrogel. Our study found that SF-SS hydrogel could be used as an excellent alternative to deliver exosomes for tissue repair.
最近的研究表明，与由单一生物材料形成的水凝胶相比，由复合生物材料形成的水凝胶是组织修复的更好选择。由于丝素蛋白（SF）具有优异的机械性能，而丝胶蛋白（SS）具有细胞粘附和生物相容性，我们探索了由丝素蛋白（SF）和丝胶蛋白（SS）形成的复合水凝胶在组织修复中的可行性。在我们的研究中，SF-SS水凝胶是通过分别提取法（SF用LiBr溶解，SS用热碱性水溶解）由SF和SS蛋白形成的，而SF-SS水凝胶是通过同时提取法（SF和SS蛋白都用LiBr溶解）由SF和SS蛋白形成的。分析了两种复合水凝胶对巨噬细胞炎症细胞因子释放和伤口的影响。此外，使用两种水凝胶封装并递送人脐带间充质干细胞衍生的外泌体（UMSC-Exo）。SF-SS和SF-SS水凝胶均促进伤口愈合、血管生成，并减少巨噬细胞的炎症和TNF-α分泌。在用封装在SF-SS水凝胶中的UMSC-Exo处理的实验组中，这些有益效果更为显著。我们的研究发现，SF-SS水凝胶可作为递送外泌体用于组织修复的极佳替代品。
