膳食纤维菊粉是否会影响葡萄柚类黄酮丰富提取物的生物活性？
Does dietary inulin affect biological activity of a grapefruit flavonoid-rich extract?
机构信息
Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
出版信息
Nutr Metab (Lond). 2012 Apr 11;9(1):31. doi: 10.1186/1743-7075-9-31.
BACKGROUND
The aim of the study was to verify that the concomitant presence of grapefruit flavonoid extract with inulin in a Western-type diet may provide synergistic effects to the hindgut metabolism, as well as blood lipid and mineral profiles.
METHODS
Forty male Wistar rats were distributed into 4 groups and fed for 28 days with diets rich in fat, cholesterol and protein. A two-way repeated measures ANOVA was applied to assess the effects of inulin (v. sucrose, 5% of the diet), the addition of dietary grapefruit flavonoid extract (diets without or with 0.3% of an extract from hard parts of grapefruit) and the interaction between these two dietary factors.
RESULTS
When compared to the control sucrose-containing diet, the diet enriched with inulin led to typical changes within the caecum, the main part of hindgut fermentation in rats, such as acidification of the digesta, support of bifidobacteria growth and increase of propionate and butyrate production. The dietary grapefruit flavonoid extract without inulin increased the bulk and pH value of caecal digesta, whereas short-chain fatty acid concentration and the bifidobacteria population were lowered compared to the extract-free diets. Simultaneous dietary addition of both tested components decreased slightly the pH value and increased somewhat the bifidobacteria number and the propionate concentration, however to the level observed with the control sucrose-containing diet. With regard to blood lipids, dietary grapefruit flavonoid extract decreased the triglyceride concentration regardless of the dietary carbohydrate type.
CONCLUSION
Inulin does not provide any additional benefit to the blood lipid profile caused by the dietary application of grapefruit flavonoid extract and it does not counteract clearly detrimental effects of the extract in the hindgut. Adding grapefruit extract to the diet must be performed with caution due to possible adverse hindgut responses with overdoses.
背景
本研究旨在验证在西方饮食中同时摄入柚皮苷类黄酮提取物和菊粉是否对后肠代谢以及血脂和矿物质谱产生协同作用。
方法
40 只雄性 Wistar 大鼠随机分为 4 组，分别用富含脂肪、胆固醇和蛋白质的饮食喂养 28 天。采用双向重复测量方差分析评估菊粉（v. 蔗糖，饮食的 5%）、添加饮食柚皮苷类黄酮提取物（饮食中有无 0.3%柚皮苷类黄酮提取物）以及这两种饮食因素相互作用的影响。
结果
与含有蔗糖的对照饮食相比，富含菊粉的饮食导致大鼠后肠发酵的主要部位——盲肠发生典型变化，如食糜酸化、双歧杆菌生长得到支持以及丙酸盐和丁酸盐产量增加。不含菊粉的饮食柚皮苷类黄酮提取物增加了盲肠食糜的体积和 pH 值，而与不含提取物的饮食相比，短链脂肪酸浓度和双歧杆菌数量降低。同时添加这两种测试成分会稍微降低 pH 值，略微增加双歧杆菌数量和丙酸盐浓度，但与含有蔗糖的对照饮食相比仍处于较低水平。就血脂而言，无论饮食中碳水化合物的类型如何，饮食柚皮苷类黄酮提取物都能降低甘油三酯浓度。
结论
菊粉对饮食应用柚皮苷类黄酮提取物引起的血脂谱没有任何额外益处，并且不能明显抵消提取物在后肠的有害影响。由于过量摄入可能会引起后肠不良反应，因此添加柚皮苷提取物到饮食中必须谨慎。
Zotero 插件