Zhai Wenliang, Wang Nan, Qi Zhi, Gao Quan, Yi Liren
Department of Orthopedic Surgery, Southeast Hospital Affiliated to Xiamen University, Zhanghou, China.
Orthopedics. 2012 Apr;35(4):e520-5. doi: 10.3928/01477447-20120327-22.
The purpose of this study was to investigate the effect of platelet-rich plasma on the proliferation of osteoblasts and tenocytes in tendon-bone healing. We cultured osteoblasts and tenocytes in an indirect coculture system with Transwell filters (Merck Millipore, Billerica, Massachusetts). The proliferation was examined using Cell Counting Kit-8 (Dojindo Chemistry Research Institute, Kumamoto, Japan).Four groups were studied: group 1, one cell type cultured without platelet-rich plasma; group 2, two cell types cultured together in an indirect coculture system without platelet-rich plasma; group 3, cells in the outer chamber and platelet-rich plasma in the inner chamber; and group 4, two different cell types in each of the 2 chambers with platelet-rich plasma in the inner chamber. The proliferation rates of groups 3 and 4 were the highest, followed by group 1 and then group 2, which was the lowest.Platelet-rich plasma abolishes the inhibition of osteoblasts or tenocytes in an indirect coculture system and improves the cell proliferation rate.
本研究的目的是探讨富血小板血浆对肌腱-骨愈合过程中成骨细胞和腱细胞增殖的影响。我们使用Transwell小室（默克密理博，马萨诸塞州比勒里卡）在间接共培养系统中培养成骨细胞和腱细胞。使用细胞计数试剂盒-8（日本熊本同仁化学研究所）检测细胞增殖情况。研究分为四组：第1组，培养一种细胞类型且不添加富血小板血浆；第2组，在间接共培养系统中共同培养两种细胞类型且不添加富血小板血浆；第3组，外室为细胞，内室为富血小板血浆；第4组，两个小室中各有两种不同细胞类型，内室含有富血小板血浆。第3组和第4组的增殖率最高，其次是第1组，第2组最低。富血小板血浆消除了间接共培养系统中对成骨细胞或腱细胞的抑制作用，并提高了细胞增殖率。
