Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, Department of Histology and Embryology in Ningxia Medical University, Yinchuan, People's Republic of China.
Reprod Biomed Online. 2012 Jun;24(6):646-53. doi: 10.1016/j.rbmo.2012.02.025. Epub 2012 Mar 10.
Ovarian tissue transplantation is now considered as a procedure to preserve the fertility of young women patients undergoing cancer therapy. The present study investigated the effects and mechanism of human menopausal gonadotrophin (HMG) intervention on vascular remoulding in ovarian heterotopic autotransplantation. Ovaries of 8-week-old mice were cultured in vitro with different concentrations of HMG for 3h for measuring the expression of vascular endothelial growth factor (VEGF). The cultured ovaries were implanted under the kidney capsule and removed 24, 36, 48 h or 1 month after transplantation. Revascularization, fluid exudation and the number of surviving ovarian follicles were observed. The results showed that VEGF was increased 1.6-6.5 times in the HMG intervention groups. Revascularization appeared 24-36 h after transplantation and was earlier than that of the control. Fluid exudation increased incrementally with increasing HMG concentrations. The total number of surviving ovarian follicles was increased by 1.2-1.5 times in the HMG 0.15 IU/ml group as compared with the other groups 1 month after transplantation. It is concluded that intervention with HMG in vitro before transplantation could improve the blood supply reconstruction and survival of the autotransplanted ovarian follicles, which might be associated with increased VEGF expression.
卵巢组织移植现在被认为是一种保存接受癌症治疗的年轻女性患者生育能力的方法。本研究探讨了人绝经期促性腺激素（HMG）干预对卵巢同种异体异位移植中血管重塑的影响及其机制。将 8 周龄小鼠的卵巢在含有不同浓度 HMG 的培养基中体外培养 3 小时，以测量血管内皮生长因子（VEGF）的表达。培养的卵巢在移植后 24、36、48 小时或 1 个月被植入肾包膜下，并进行观察。观察血管重铸、液体渗出和存活的卵巢卵泡数量。结果表明，HMG 干预组的 VEGF 表达增加了 1.6-6.5 倍。血管重铸在移植后 24-36 小时出现，早于对照组。随着 HMG 浓度的增加，液体渗出逐渐增加。与其他组相比，HMG 0.15IU/ml 组在移植后 1 个月时存活的卵巢卵泡总数增加了 1.2-1.5 倍。结论：移植前体外 HMG 干预可改善自体移植卵巢卵泡的血供重建和存活，这可能与 VEGF 表达增加有关。
