Chang Yung-Ching, Liu Chan-Chuan, Chan Wan-Ping, Lin Yu-Long, Sze Chun-I, Chen Shiuan-Yeh
Department of Photonics, National Cheng Kung University, Tainan City, 70101, Taiwan.
Department of Cell Biology and Anatomy, National Cheng Kung University, Tainan City 70101, Taiwan.
ACS Appl Bio Mater. 2025 Apr 21;8(4):3119-3126. doi: 10.1021/acsabm.4c01986. Epub 2025 Mar 18.
Glioblastoma (GBM) is an aggressive and fatal tumor. The infiltrative spread of GBM cells hinders gross total resection. The residual GBM cells are significantly associated with survival and recurrence. Therefore, a theranostic method that can enhance the contrast between residual GBM and normal astrocyte (AS) cells and selectively eradicate GBM cells is highly desired. In this report, GBM and normal astrocyte cells are both cultured in the same microplate well to imitate a coexistence environment and treated with Raman tags functionalized by anti-EGFR. Compared to AS cells, GBM cells show 25% higher Raman emission, and their cell death rate increases by a factor of 2. These results demonstrate the potential for selective eradication of the residual GBM cells guided by robust Raman signals after the primary GBM surgery.
胶质母细胞瘤（GBM）是一种侵袭性致命肿瘤。GBM细胞的浸润性扩散阻碍了肿瘤的全切。残留的GBM细胞与患者的生存和复发密切相关。因此，人们迫切需要一种能够增强残留GBM细胞与正常星形胶质细胞（AS）之间对比度并选择性根除GBM细胞的诊疗方法。在本报告中，将GBM细胞和正常星形胶质细胞共同培养于同一微孔板孔中以模拟共存环境，并用抗表皮生长因子受体（EGFR）功能化的拉曼标签进行处理。与AS细胞相比，GBM细胞的拉曼发射高25%，其细胞死亡率增加了一倍。这些结果表明，在原发性GBM手术后，利用强大的拉曼信号引导选择性根除残留GBM细胞具有潜力。
