Flancbaum L, Brotman D N, Fitzpatrick J C, Van Es T, Kasziba E, Fisher H
Dept. of Surgery, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey.
Life Sci. 1990;47(17):1587-93. doi: 10.1016/0024-3205(90)90188-w.
Carcinine (beta-alanylhistamine) was synthesized in vitro from histamine and beta-alanine. It was detected quantitatively using an HPLC method previously described for the quantification of the related compounds histamine, histidine, carnosine and 3-methylhistamine. Carcinine was identified in several tissue of the rat, guinea pig, mouse and human, and was then shown to be metabolically related in vivo to histamine, histidine, carnosine and 3-methylhistamine through radioisotopic labeling. The results demonstrate that carcinine may be concurrently quantitated using the same HPLC method as that used to measure histamine, histidine, carnosine and 3-methylhistamine. These findings suggest a role for carcinine in the carnosine-histidine-histamine metabolic pathway and in the mammalian physiologic response to stress.
肌肽（β-丙氨酰组胺）在体外由组胺和β-丙氨酸合成。使用先前描述的用于定量相关化合物组胺、组氨酸、肌肽和3-甲基组胺的高效液相色谱法对其进行定量检测。在大鼠、豚鼠、小鼠和人类的多种组织中鉴定出了肌肽，然后通过放射性同位素标记表明其在体内与组胺、组氨酸、肌肽和3-甲基组胺存在代谢关联。结果表明，可使用与测量组胺、组氨酸、肌肽和3-甲基组胺相同的高效液相色谱法同时对肌肽进行定量。这些发现提示肌肽在肌肽-组氨酸-组胺代谢途径以及哺乳动物对应激的生理反应中发挥作用。
