Department of Chemical Engineering, Queen's University, Kingston, Ontario, Canada.
Biomacromolecules. 2012 May 14;13(5):1478-85. doi: 10.1021/bm300186k. Epub 2012 Apr 24.
Wound healing is a natural process involving several signaling molecules and cell types over a significant period of time. Although current dressings help to protect the wound from debris or infection, they do little in accelerating the healing process. Insulin has been shown to stimulate the healing of damaged skin. We have developed an alginate sponge dressing (ASD) that forms a hydrogel capable of providing a moist and protective healing environment. By incorporating insulin-loaded poly(d,l-lactide-co-glycolide) (PLGA) microparticles into ASD, we successfully stabilized and released insulin for up to 21 days. Insulin release and water absorption and transfer through the ASD were influenced by altering the levels of poly(ethylene glycol) (PEG) in the dressing matrix. Bioactivity of released insulin can be maintained for at least 10 days, demonstrated using a human keratinocyte migration assay. Results showed that insulin-loaded PLGA microparticles, embedded within PEG-ASD, functioned as an effective long-term delivery platform for bioactive insulin.
伤口愈合是一个自然的过程，涉及到多个信号分子和细胞类型，需要经过相当长的一段时间。虽然目前的敷料有助于保护伤口免受碎屑或感染，但它们在加速愈合过程方面作用甚微。胰岛素已被证明可以刺激受损皮肤的愈合。我们开发了一种藻酸盐海绵敷料 (ASD)，它形成水凝胶，能够提供湿润和保护的愈合环境。通过将负载胰岛素的聚（D，L-乳酸-共-乙醇酸）（PLGA）微球掺入 ASD 中，我们成功地稳定并释放了长达 21 天的胰岛素。通过改变敷料基质中聚乙二醇（PEG）的水平，可以影响胰岛素的释放和水的吸收以及通过 ASD 的转移。通过人角质形成细胞迁移试验证明，释放的胰岛素的生物活性至少可以维持 10 天。结果表明，负载胰岛素的 PLGA 微球嵌入 PEG-ASD 中，可作为生物活性胰岛素的有效长效递送平台。
