Cross S, Lindsey J, Fantes J, McKay S, McGill N, Cooke H
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Nucleic Acids Res. 1990 Nov 25;18(22):6649-57. doi: 10.1093/nar/18.22.6649.
All telomeres which have been studied consist of an array of simple G/C rich repeats. Human telomeres were shown to share sequence similarity with those of lower eukaryotes by cross-hybridization and human telomeric sequences have been cloned by complementation of telomere function in yeast. Analysis of human telomeric sequences cloned in this way is described here. The terminal part of the cloned human telomeric DNA consists of an array of simple repeats, principally of the sequence TTAGGG and derivatives. The very terminal part consists of yeast-type telomeric repeats which suggests that the human telomeric sequences have acted as a primer for the addition of additional telomeric repeats in the yeast. Subterminal sequences are shared between a number of clones and in situ data shows that these subterminal sequences are present at several different chromosomal ends. Related sequences are present at internal as well as telomeric positions. Differences in the hybridization patterns of subterminal sequences in somatic compared to germ-line tissues are described which indicate differential modification of these sequences during development.
所有已被研究的端粒都由一系列富含G/C的简单重复序列组成。通过交叉杂交发现，人类端粒与低等真核生物的端粒具有序列相似性，并且人类端粒序列已通过酵母中端粒功能的互补作用被克隆出来。本文描述了以此方式克隆的人类端粒序列的分析。克隆的人类端粒DNA的末端部分由一系列简单重复序列组成，主要是TTAGGG序列及其衍生物。最末端部分由酵母型端粒重复序列组成，这表明人类端粒序列在酵母中充当了添加额外端粒重复序列的引物。多个克隆之间共享亚端粒序列，原位数据表明这些亚端粒序列存在于几个不同的染色体末端。相关序列在内部位置以及端粒位置均有存在。文中描述了体细胞与生殖系组织中亚端粒序列杂交模式的差异，这表明这些序列在发育过程中发生了差异修饰。
