Technical Research Laboratory, Takanashi Milk Products Co. Ltd., Yokohama, Kanagawa, Japan.
J Appl Microbiol. 2012 Jul;113(1):155-62. doi: 10.1111/j.1365-2672.2012.05316.x. Epub 2012 May 15.
To investigate the influence of heat-killed Lactobacillus gasseri TMC0356 on changes in respiratory immune function and intestinal microbiota in a diet-induced obese mouse model.
Male C57BL/6J mice were fed a high-fat diet for 16 weeks. After 8 weeks, the high-fat-diet-induced obese mice (DIO mice) were randomly divided into two 0067roups, the DIO and DIO0356 groups. DIO0356 group mice were orally fed with heat-killed TMC0356 every day for 8 weeks, while DIO group mice were exposed to 0·85% NaCl over the same time period as controls. After intervention, the pulmonary mRNA expression of cytokines and other immune molecules in DIO0356 mice compared to those in DIO group mice was significantly increased (P < 0·05, P < 0·01). In faecal bacterial profiles, analysed using the terminal restriction fragment length polymorphism (T-RFLP) method, T-RFLP patterns in 75% of the DIO0356 group mice were apparently changed compared with those in control group mice.
These results suggest that inactive lactobacilli may stimulate the respiratory immune responses of obese host animals to enhance their natural defences against respiratory infection, partially associating with their potent impact on intestinal microbiota.
We have demonstrated that oral administration of inactive lactobacilli may protect host animals from the lung immune dysfunction caused by obesity.
研究热灭活格氏乳杆菌 TMC0356 对饮食诱导肥胖小鼠模型呼吸免疫功能和肠道微生物群变化的影响。
雄性 C57BL/6J 小鼠喂食高脂肪饮食 16 周。8 周后，将高脂肪饮食诱导的肥胖小鼠（DIO 小鼠）随机分为两组，DIO 和 DIO0356 组。DIO0356 组小鼠每天口服热灭活 TMC0356 持续 8 周，而 DIO 组小鼠在相同时间内暴露于 0.85%NaCl 作为对照。干预后，与 DIO 组小鼠相比，DIO0356 组小鼠肺部细胞因子和其他免疫分子的 mRNA 表达明显增加（P<0.05，P<0.01）。在粪便细菌谱分析中，采用末端限制性片段长度多态性（T-RFLP）方法，75%的 DIO0356 组小鼠的 T-RFLP 图谱明显改变，与对照组小鼠相比。
这些结果表明，无活性乳酸菌可能会刺激肥胖宿主动物的呼吸免疫反应，增强其对呼吸道感染的天然防御能力，这与它们对肠道微生物群的强烈影响部分相关。
我们已经证明，口服无活性乳酸菌可能会保护宿主动物免受肥胖引起的肺部免疫功能障碍。
