Nuclear and Radiological Engineering Program, The George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Nat Methods. 2012 Apr 22;9(7):721-3. doi: 10.1038/nmeth.1978.
In super-resolution microscopy methods based on single-molecule switching, the rate of accumulating single-molecule activation events often limits the time resolution. Here we developed a sparse-signal recovery technique using compressed sensing to analyze images with highly overlapping fluorescent spots. This method allows an activated fluorophore density an order of magnitude higher than what conventional single-molecule fitting methods can handle. Using this method, we demonstrated imaging microtubule dynamics in living cells with a time resolution of 3 s.
在基于单分子开关的超分辨率显微镜方法中，累积单分子激活事件的速率通常会限制时间分辨率。在这里，我们开发了一种使用压缩感知的稀疏信号恢复技术来分析荧光斑点高度重叠的图像。这种方法可以使激活荧光团的密度比传统的单分子拟合方法高一个数量级。使用这种方法，我们展示了在活细胞中以 3 秒的时间分辨率成像微管动力学的实验结果。
