利用酵母对淀粉样蛋白的研究。
Study of amyloids using yeast.
作者信息
Wickner Reed B, Kryndushkin Dmitry, Shewmaker Frank, McGlinchey Ryan, Edskes Herman K
机构信息
Laboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2012;849:321-46. doi: 10.1007/978-1-61779-551-0_22.
Abstract
We detail some of the genetic, biochemical, and physical methods useful in studying amyloids in yeast, particularly the yeast prions. These methods include cytoduction (cytoplasmic mixing), infection of cells with prion amyloids, use of green fluorescent protein fusions with amyloid-forming proteins for cytology, protein purification and amyloid formation, and electron microscopy of filaments.
摘要
我们详细介绍了一些在酵母中研究淀粉样蛋白，特别是酵母朊病毒时有用的遗传、生化和物理方法。这些方法包括细胞导入（细胞质混合）、用朊病毒淀粉样蛋白感染细胞、将绿色荧光蛋白与形成淀粉样蛋白的蛋白质融合用于细胞学研究、蛋白质纯化和淀粉样蛋白形成，以及丝状结构的电子显微镜观察。
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