Department of Biotechnology, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
Appl Biochem Biotechnol. 2012 Jul;167(6):1763-77. doi: 10.1007/s12010-012-9578-y. Epub 2012 Apr 24.
Although it is known that Palk Bay sediments harbor diverse and novel bacteria with important ecological and environmental functions, a comprehensive view of their molecular diversity is still lacking. In the present study, bacterial diversity in Palk Bay sediments was characterized using the molecular method terminal-restriction fragment length polymorphisms (T-RFLP). The bacterial assemblages detected by T-RFLP analysis revealed that the nearshore sediment harbored high number of bacterial count, whereas the 2.5-m sediment harbored diverse and distinct bacterial composition with fine heterogeneity. The major bacterial groups detected in all the three sediment samples were Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria (including alpha (α), gamma (γ), delta (δ), and epsilon (ε)-Proteobacteria), and uncultured bacteria. This is the first study that reveals the presence of Bacteroidetes, delta (δ)- and epsilon (ε)-Proteobacteria, and uncultured bacteria in Palk Bay sediments. The hitherto unexplored wide microbial diversity of Palk Bay coastal area was unraveled in the current study through culture-independent approach. These data suggest that the continued use of cultivation-independent techniques will undoubtedly lead to the discovery of additional bacterial diversity and provide a direct means to learn more about the ecophysiology and biotechnological potential of Palk Bay coastal area.
尽管已知帕尔克湾沉积物中蕴藏着具有重要生态和环境功能的多样而新颖的细菌，但对其分子多样性的全面了解仍然缺乏。在本研究中，使用分子方法末端限制性片段长度多态性（T-RFLP）对帕尔克湾沉积物中的细菌多样性进行了表征。T-RFLP 分析检测到的细菌组合表明，近岸沉积物中细菌数量较多，而 2.5 米深的沉积物具有多样而独特的细菌组成，具有精细的异质性。在所有三个沉积物样本中检测到的主要细菌群是放线菌、拟杆菌门、厚壁菌门、变形菌门（包括α（α）、γ（γ）、δ（δ）和ε（ε）-变形菌门）和未培养细菌。这是首次在帕尔克湾沉积物中发现拟杆菌门、δ（δ）-和ε（ε）-变形菌门和未培养细菌的研究。本研究通过非培养方法揭示了帕尔克湾沿海地区以前未知的广泛微生物多样性。这些数据表明，继续使用非培养技术无疑将导致发现更多的细菌多样性，并提供一种直接的方法来更多地了解帕尔克湾沿海地区的生态生理学和生物技术潜力。
