2型糖尿病患者与健康个体粪便中双歧杆菌种类的差异
[Differences in fecal Bifidobacterium species between patients with type 2 diabetes and healthy individuals].
作者信息
Xu Xiaojin, Hui Hongxiang, Cai Dehong
机构信息
Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Apr;32(4):531-3, 564.
OBJECTIVE
To determine the changes in fecal Bifidobacterium species in patients with type 2 diabetes in comparison with healthy individuals.
METHODS
The bacterial DNA were extracted from the fecal samples from 50 type 2 diabetic patients and 30 healthy individuals. Real-time quantitative PCR was employed to determine the copy numbers of the bacteria in the fecal samples using 16S rRNA-targeted genus- and species-specific PCR primers for a selected group of fecal Bifidobacterium species including total Bifidobacterium, B.longum, B.breve, B.adolescent, and B. infantis.
RESULTS
The diabetic group had significantly lower copy numbers of total Bifidobacterium and B.adolescent compared to the healthy individuals (P<0.05).
CONCLUSION
Type 2 diabetic patients have a lowered number of Bifidobacterium species in the gut microflora.
目的
与健康个体相比，确定2型糖尿病患者粪便中双歧杆菌种类的变化。
方法
从50例2型糖尿病患者和30例健康个体的粪便样本中提取细菌DNA。使用针对一组选定的粪便双歧杆菌种类（包括总双歧杆菌、长双歧杆菌、短双歧杆菌、青春双歧杆菌和婴儿双歧杆菌）的16S rRNA靶向属特异性和种特异性PCR引物，采用实时定量PCR来确定粪便样本中细菌的拷贝数。
结果
与健康个体相比，糖尿病组总双歧杆菌和青春双歧杆菌的拷贝数显著降低（P<0.05）。
结论
2型糖尿病患者肠道微生物群中双歧杆菌种类数量减少。
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