粪便中人类轮状病毒的常规诊断。
Routine diagnosis of human rotaviruses in stools.
作者信息
Zissis G, Lambert J P, De Kegel D
出版信息
J Clin Pathol. 1978 Feb;31(2):175-8. doi: 10.1136/jcp.31.2.175.
Abstract
Electron microscopy, immune electron microscopy and complement fixation as methods of detecting rotavirus in the stools of young children with gastroenteritis were compared in a blind study during the winter of 1975-6. Complement fixation was the simplest to perform, was as sensitive as the other two, and allowed a quantitative measurement of viral excretion. Absorption of faecal extracts with fetal calf serum usually removed the anticomplementary activity of faecal extracts.
摘要
1975 - 1976年冬季，在一项盲法研究中，对电子显微镜检查、免疫电子显微镜检查和补体结合试验这三种检测患肠胃炎幼儿粪便中轮状病毒的方法进行了比较。补体结合试验操作最简单，与其他两种方法一样灵敏，并且能够对病毒排泄进行定量测定。用胎牛血清吸收粪便提取物通常可消除粪便提取物的抗补体活性。
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