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粪便中人类轮状病毒的常规诊断。

Routine diagnosis of human rotaviruses in stools.

作者信息

Zissis G, Lambert J P, De Kegel D

出版信息

J Clin Pathol. 1978 Feb;31(2):175-8. doi: 10.1136/jcp.31.2.175.

DOI:10.1136/jcp.31.2.175
PMID:204665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1145212/
Abstract

Electron microscopy, immune electron microscopy and complement fixation as methods of detecting rotavirus in the stools of young children with gastroenteritis were compared in a blind study during the winter of 1975-6. Complement fixation was the simplest to perform, was as sensitive as the other two, and allowed a quantitative measurement of viral excretion. Absorption of faecal extracts with fetal calf serum usually removed the anticomplementary activity of faecal extracts.

摘要

1975 - 1976年冬季,在一项盲法研究中,对电子显微镜检查、免疫电子显微镜检查和补体结合试验这三种检测患肠胃炎幼儿粪便中轮状病毒的方法进行了比较。补体结合试验操作最简单,与其他两种方法一样灵敏,并且能够对病毒排泄进行定量测定。用胎牛血清吸收粪便提取物通常可消除粪便提取物的抗补体活性。

相似文献

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Routine diagnosis of human rotaviruses in stools.粪便中人类轮状病毒的常规诊断。
J Clin Pathol. 1978 Feb;31(2):175-8. doi: 10.1136/jcp.31.2.175.
2
[Demonstration of rotaviruses in feces by means of the complement fixation test].
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3
Isolation of a reovirus-like agent (rotavirus) from neonatal calf diarrhea in Japan.
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J Med Virol. 1978;2(1):21-8. doi: 10.1002/jmv.1890020105.
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[Detection of an agent similar to rotavirus in infantile gastroenteritis].[婴儿肠胃炎中类似轮状病毒病原体的检测]
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Rotavirus in Buenos Aires, Argentina.阿根廷布宜诺斯艾利斯的轮状病毒。
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Epidemiological and virological studies on outbreaks of acute gastroenteritis associated with rotavirus in primary schools in Osaka.大阪市小学中与轮状病毒相关的急性胃肠炎暴发的流行病学和病毒学研究
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J Med Virol. 1977;1(1):69-77. doi: 10.1002/jmv.1890010109.
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[Existence of 2 types of Rotavirus associated with acute gastroenteritis in children].[与儿童急性胃肠炎相关的两种轮状病毒的存在]
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Simplified radioimmunoassay for detection of human rotavirus in stools.用于检测粪便中人类轮状病毒的简易放射免疫测定法。
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引用本文的文献

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Detection of rotavirus antibody by inhibition of reverse passive hemagglutination.
J Clin Microbiol. 1982 Jan;15(1):148-55. doi: 10.1128/jcm.15.1.148-155.1982.
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Viral gastroenteritis.病毒性肠胃炎
Med Clin North Am. 1983 Sep;67(5):1031-58. doi: 10.1016/s0025-7125(16)31165-8.
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Human rotavirus detection by agglutination of antibody-coated erythrocytes.通过抗体包被红细胞凝集法检测人轮状病毒
J Clin Microbiol. 1983 Jun;17(6):1141-7. doi: 10.1128/jcm.17.6.1141-1147.1983.
6
Epidemiology of human rotaviruses as determined by electrophoresis of genome RNA.通过基因组RNA电泳确定的人类轮状病毒流行病学
Eur J Clin Microbiol. 1983 Apr;2(2):141-7. doi: 10.1007/BF02001580.
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Comparison of ELISA, SPACE, and electron microscopy for the routine diagnosis of rotavirus infection.酶联免疫吸附测定法、固相抗体捕捉酶免疫测定法及电子显微镜用于轮状病毒感染常规诊断的比较
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Frequency of preclumped virus in routine fecal specimens from patients with acute nonbacterial gastroenteritis.急性非细菌性肠胃炎患者常规粪便样本中预聚集病毒的频率。
J Clin Microbiol. 1981 May;13(5):982-8. doi: 10.1128/jcm.13.5.982-988.1981.
9
Diagnosis of human rotavirus infections: comparison of an electrophoretic method, a modified complement fixation test and electron microscopy for rotavirus detection.人类轮状病毒感染的诊断:用于轮状病毒检测的电泳法、改良补体结合试验和电子显微镜检查的比较
Arch Virol. 1981;67(4):341-4. doi: 10.1007/BF01314837.
10
Rotavirus and other viruses in the stool of premature babies.早产儿粪便中的轮状病毒及其他病毒。
J Med Virol. 1980;5(2):137-42. doi: 10.1002/jmv.1890050207.

本文引用的文献

1
Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests.粪便中的呼肠孤病毒样病原体:与婴儿腹泻的关联及血清学检测的发展
Science. 1974 Sep 20;185(4156):1049-53. doi: 10.1126/science.185.4156.1049.
2
Letter: "Rotavirus" in gastroenteritis.信函:胃肠炎中的“轮状病毒”
Lancet. 1974 Sep 21;2(7882):726. doi: 10.1016/s0140-6736(74)93308-x.
3
Letter: "Rotavirus" in stored specimens of faecal extracts.信件:粪便提取物储存样本中的“轮状病毒”
Lancet. 1974 Nov 2;2(7888):1083. doi: 10.1016/s0140-6736(74)92187-4.
4
Letter: Orbiviruses and gastroenteritis.信函:环状病毒与肠胃炎
Lancet. 1974 Sep 14;2(7881):658-9. doi: 10.1016/s0140-6736(74)91987-4.
5
Letter: Virus in faecal extracts from children with gastroenteritis.信件:来自肠胃炎患儿粪便提取物中的病毒。
Lancet. 1974 Jun 1;1(7866):1109. doi: 10.1016/s0140-6736(74)90584-4.
6
Letter: Viruses in gastroenteritis.信件:肠胃炎中的病毒。
Lancet. 1974 Jun 29;1(7870):1353. doi: 10.1016/s0140-6736(74)90738-7.
7
Letter: Viral-antibody detection by a more sensitive complement-fixation reaction.信件:通过更灵敏的补体结合反应进行病毒抗体检测。
Lancet. 1974 Apr 20;1(7860):754-5. doi: 10.1016/s0140-6736(74)92982-1.
8
Letter: Virus particles in gastroenteritis.信函:肠胃炎中的病毒颗粒。
Lancet. 1973 Dec 29;2(7844):1497. doi: 10.1016/s0140-6736(73)92760-8.
9
Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis.急性非细菌性胃肠炎患儿十二指肠黏膜上皮细胞中的病毒颗粒。
Lancet. 1973 Dec 8;2(7841):1281-3. doi: 10.1016/s0140-6736(73)92867-5.
10
Letter: Occurrence of "viral particles" in diarrhea: Atlanta, Georgia.信函:佐治亚州亚特兰大市腹泻病例中“病毒颗粒”的出现情况
N Engl J Med. 1975 Jun 12;292(24):1297. doi: 10.1056/NEJM197506122922416.