电子显微镜检查、酶联免疫吸附测定、固相放射免疫测定及间接免疫荧光法检测粪便中人类轮状病毒抗原的比较
Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.
作者信息
Birch C J, Lehmann N I, Hawker A J, Marshall J A, Gust I D
出版信息
J Clin Pathol. 1979 Jul;32(7):700-5. doi: 10.1136/jcp.32.7.700.
Abstract
Four techniques were compared for their practicability, speed, and sensitivity for the detection of human rotavirus. Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) were found to be the most sensitive means of identifying rotavirus, and, once processed, up to 40 specimens could be examined daily. Electron microscopy, although less sensitive than these techniques, had the advantage of being able to detect other viral agents present in faecal extracts. Indirect immunofluorescence failed to detect rotavirus as often as the other three methods. In laboratories where routine examination of faecal specimens from patients with gastroenteritis is required, ELISA and RIA are useful alternatives to electron microscopy.
摘要
对检测人轮状病毒的四种技术在实用性、速度和灵敏度方面进行了比较。放射免疫测定法（RIA）和酶联免疫吸附测定法（ELISA）被发现是鉴定轮状病毒最灵敏的方法，且一旦处理完成，每天最多可检测40份标本。电子显微镜检查虽然不如这些技术灵敏，但具有能够检测粪便提取物中存在的其他病毒病原体的优势。间接免疫荧光法检测轮状病毒的能力不如其他三种方法。在需要对肠胃炎患者粪便标本进行常规检查的实验室中，ELISA和RIA是电子显微镜检查的有用替代方法。
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