Department of Health, Nutrition and Management, Faculty of Health Sciences, Oslo and Akershus University College of Applied Sciences, PO Box 4, St Olavs Plass, NO-0130 Oslo, Norway.
Food Chem Toxicol. 2012 Jul;50(7):2462-72. doi: 10.1016/j.fct.2012.04.026. Epub 2012 Apr 21.
Blue mussels (Mytilus edulis) accumulate and biotransform arsenic (As) to a larger variety of arsenicals than most seafood. Eight volunteers ingested a test meal consisting of 150 g blue mussel (680 μg As), followed by 72 h with an identical, low As controlled diet and full urine sampling. We provide a complete speciation, with individual patterns, of urinary As excretion. Total As (tAs) urinary excretion was 328 ± 47 μg, whereof arsenobetaine (AB) and dimethylarsinate (DMA) accounted for 66% and 21%, respectively. Fifteen minor urinary arsenicals were quantified with inductively coupled plasma mass spectrometry (ICPMS) coupled to reverse-phase, anion and cation-exchange high performance liquid chromatography (HPLC). Thio-arsenicals and non-thio minor arsenicals (including inorganic As (iAs) and methylarsonate (MA)) contributed 10% and 7% of the total sum of species excretion, respectively, but there were large individual differences in the excretion patterns. Apparently, formation of thio-arsenicals was negatively correlated to AB formation and excretion, possibly indicating a metabolic interrelationship. The results may be of toxicological relevance since DMA and MA have been classified as possibly carcinogenic, and six of the excreted As species were thio-arsenicals which recently have been recognized as toxic, while iAs toxicity is well known.
贻贝（Mytilus edulis）比大多数海鲜能更大量地积累和生物转化砷（As），形成更多种类的砷化合物。8 名志愿者摄入了一份由 150 克贻贝（680μg As）组成的测试餐，随后 72 小时内食用相同的低砷对照饮食，并进行完整的尿液采样。我们提供了完整的尿液砷排泄形态分析，包括个体形态。总砷（tAs）的尿排泄量为 328±47μg，其中砷甜菜碱（AB）和二甲基砷酸（DMA）分别占 66%和 21%。使用电感耦合等离子体质谱（ICPMS）结合反相、阴离子和阳离子交换高效液相色谱（HPLC）定量了 15 种微量尿液砷化合物。硫代砷化合物和非硫代微量砷化合物（包括无机砷（iAs）和甲基砷酸盐（MA））分别占总排泄物种的 10%和 7%，但个体之间的排泄模式存在很大差异。显然，硫代砷化合物的形成与 AB 的形成和排泄呈负相关，这可能表明存在代谢相互关系。这些结果可能具有毒理学意义，因为 DMA 和 MA 被归类为可能致癌物质，而排泄的 6 种砷化合物是最近被认为有毒的硫代砷化合物，而 iAs 的毒性众所周知。
