Takeuchi Shouhei, Katoh Takahiko, Yamauchi Takenori, Kuroda Yoshiki
Department of Public Health, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
Exp Diabetes Res. 2012;2012:973561. doi: 10.1155/2012/973561. Epub 2012 Apr 8.
The aim of this study was to evaluate the association between the Trp64Arg polymorphism in the beta3-adrenergic receptor gene (ADRB3: rs4994) and BMI and serological and anthropometric data in healthy Japanese.
Healthy Japanese recruited in a large-scale integrated manufacturing facility in Japan (N = 1355; age: 37.25 ± 9.43; BMI: 22.86 ± 3.46) were eligible for analysis. The anthropometric data and serological data were measured during a comprehensive health check, and a self-reporting questionnaire was used to assess lifestyle habits (current exercise, smoking status, alcohol intake, and working style) and weight at age 20. Genotyping for the ADRB3 polymorphism was performed by PCR-RFLP method.
Among 1355 participants, the genotype frequencies of the Trp/Trp, Trp/Arg, and Arg/Arg variants were 920 (67.9%), 394 (29.1%), and 41 (3.05%), respectively. In the multivariate analysis, a multiple linear regression model in men for the adjustment of age, drinking habits, smoking habits, exercise habits, working status and serological measurements statistically showed an overall weak significance between annual BMI gain from age 20 and age, LDL or ADRB3 polymorphism.
The level of LDL, age, and ADRB3 polymorphism (Arg/Arg genotype) were statistically associated with annual BMI gain in Japanese men.
本研究旨在评估β3 - 肾上腺素能受体基因（ADRB3：rs4994）中的色氨酸64精氨酸多态性与健康日本人的体重指数（BMI）以及血清学和人体测量数据之间的关联。
在日本一家大型综合制造工厂招募的健康日本人（N = 1355；年龄：37.25 ± 9.43；BMI：22.86 ± 3.46）符合分析条件。在全面健康检查期间测量人体测量数据和血清学数据，并使用自我报告问卷评估生活习惯（当前运动、吸烟状况、饮酒量和工作方式）以及20岁时的体重。通过聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）方法对ADRB3多态性进行基因分型。
在1355名参与者中，Trp/Trp、Trp/Arg和Arg/Arg变体的基因型频率分别为920（67.9%）、394（29.1%）和41（3.05%）。在多变量分析中，男性的多元线性回归模型用于调整年龄、饮酒习惯、吸烟习惯、运动习惯、工作状态和血清学测量值，结果在统计学上显示，从20岁到现在的年度BMI增加与年龄、低密度脂蛋白（LDL）或ADRB3多态性之间总体存在较弱的显著性。
在日本男性中，LDL水平、年龄和ADRB3多态性（Arg/Arg基因型）与年度BMI增加在统计学上相关。
