Aycicek Abdullah, Çetinkaya Zafer, Kıyıcı Halil, Bukulmez Aysegul, Yucedag Fatih
Department of Otolaryngology, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
Int J Pediatr Otorhinolaryngol. 2012 Aug;76(8):1087-90. doi: 10.1016/j.ijporl.2012.04.005. Epub 2012 Apr 30.
To investigate whether Helicobacter pylori causes inflammation in the normal middle ear and in the middle ear with effusion.
Sixteen adult New Zealand white rabbits were divided into two gropus equally. Group I was representing histamine-induced middle ear inflammation and Group II was representing normal middle ear. While H. pylori was inoculated in the right ears, physiologic saline was inoculated in the left ears of the rabbits in both groups. Results were evaluated clinically, histopathologically and microbiologically. Fisher's exact test was used for statistical analysis.
In Group I, clinical scores of the inflammation in the right ears were higher than the left ears scores at the 7th day. Histopathological scores of the inflammation in the right ears were higher than the left ears scores at the 7th day. Also, H. pylori was isolated in 6 of the 8 right ears of the rabbits. In Group II, while clinical scores of the inflammation in the right ears scores were higher than the left ears scores at the 7th day, histopathological scores were not significantly different between both ears. Also, no H. pylori was isolated in right ears of the rabbits.
Effusion in the middle ear induced by histamine is an appropriate medium for H. pylori reproduction and it also aggravates the inflammation process. In contrary, H. pylori did not cause inflammation in the normal middle ear. We suggest that H. pylori does not play a role in the etiology of otitis media with effusion alone, but it contributes to the inflammation process in the presence of an effusion.
研究幽门螺杆菌是否会在正常中耳及中耳积液中引发炎症。
将16只成年新西兰白兔平均分为两组。第一组代表组胺诱导的中耳炎症，第二组代表正常中耳。两组兔子的右耳接种幽门螺杆菌，左耳接种生理盐水。从临床、组织病理学和微生物学方面评估结果。采用Fisher精确检验进行统计分析。
在第一组中，第7天时右耳炎症的临床评分高于左耳评分。第7天时右耳炎症的组织病理学评分高于左耳评分。此外，8只兔子的右耳中有6只分离出幽门螺杆菌。在第二组中，第7天时右耳炎症的临床评分高于左耳评分，但双耳的组织病理学评分无显著差异。此外，兔子的右耳未分离出幽门螺杆菌。
组胺诱导的中耳积液是幽门螺杆菌繁殖的适宜介质，且会加剧炎症过程。相反，幽门螺杆菌不会在正常中耳中引发炎症。我们认为，幽门螺杆菌并非单独在中耳积液性中耳炎的病因中起作用，但在存在积液的情况下会促进炎症过程。
