小鼠乳腺癌细胞中病毒特异性RNA翻译的证据。
Evidence for translation of viral-specific RNA in cells of a mouse mammary carcinoma.
作者信息
Axel R, Schlom J, Spiegelman S
出版信息
Proc Natl Acad Sci U S A. 1972 Mar;69(3):535-8. doi: 10.1073/pnas.69.3.535.
Abstract
A procedure is described permitting the detection of viral-specific RNA in a mouse mammary tumor. The method involves molecular hybridization with radioactively labeled DNA complementary to the RNA of the mouse mammary-tumor virus. RNA homologous to that of the mammary agent has been found in both the nuclear and polyribosomal fractions of tumor cells. The results imply that the oncogenic information is serving as messenger RNA that directs the synthesis of proteins required for virus production, and perhaps for the maintenance of the neoplastic state. The technology developed is immediately applicable to tumors of human origin.
摘要
本文描述了一种用于检测小鼠乳腺肿瘤中病毒特异性RNA的方法。该方法涉及用与小鼠乳腺肿瘤病毒RNA互补的放射性标记DNA进行分子杂交。在肿瘤细胞的核和多核糖体部分均发现了与乳腺病原体RNA同源的RNA。结果表明，致癌信息作为信使RNA指导病毒产生所需蛋白质的合成，或许也指导维持肿瘤状态所需蛋白质的合成。所开发的技术可立即应用于人类起源的肿瘤。
相似文献
1
Evidence for translation of viral-specific RNA in cells of a mouse mammary carcinoma.小鼠乳腺癌细胞中病毒特异性RNA翻译的证据。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):535-8. doi: 10.1073/pnas.69.3.535.
2
Human sarcomas contain RNA related to the RNA of a mouse leukemia virus.人类肉瘤含有与小鼠白血病病毒RNA相关的RNA。
Science. 1972 Jan 14;175(4018):182-5. doi: 10.1126/science.175.4018.182.
3
RNA in human leukemic cells related to the RNA of a mouse leukemia virus (leukocytes-RNA-DNA hybridization-rauscher virus-polysomal RNA).人类白血病细胞中的RNA与小鼠白血病病毒的RNA相关（白细胞-RNA-DNA杂交-劳斯氏病毒-多聚核糖体RNA）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):435-9. doi: 10.1073/pnas.69.2.435.
4
RNA related to that of a murine leukemia virus in Burkitt's tumors and nasopharyngeal carcinomas.与伯基特淋巴瘤和鼻咽癌中鼠白血病病毒相关的RNA。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):5-9. doi: 10.1073/pnas.70.1.5.
5
The relationship of visna, maedi and RNA tumor viruses as studied by molecular hybridization.通过分子杂交研究维斯纳病、梅迪病与RNA肿瘤病毒之间的关系。
Virology. 1973 Mar;52(1):287-91. doi: 10.1016/0042-6822(73)90418-2.
6
Presence in human breast cancer of RNA homologous to mouse mammary tumour virus RNA.人类乳腺癌中存在与小鼠乳腺肿瘤病毒RNA同源的RNA。
Nature. 1972 Jan 7;235(5332):32-6. doi: 10.1038/235032a0.
7
Virus-related RNA in human and mouse mammary tumors.人类和小鼠乳腺肿瘤中与病毒相关的RNA。
J Natl Cancer Inst. 1972 Apr;48(4):1205-11.
8
Simultaneous detection of reverse transcriptase and high molecular weight RNA unique to oncogenic RNA viruses.同时检测致癌RNA病毒特有的逆转录酶和高分子量RNA。
Science. 1971 Nov 19;174(4011):840-3. doi: 10.1126/science.174.4011.840.
9
Detection of RNA-instructed DNA polymerase and high molecular weight RNA in malignant tissue.恶性组织中RNA指导的DNA聚合酶和高分子量RNA的检测
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2020-4. doi: 10.1073/pnas.69.8.2020.
10
The relevance of RNA tumor viruses to human cancer.RNA肿瘤病毒与人类癌症的相关性。
Johns Hopkins Med J Suppl. 1973;2:282-312.
引用本文的文献
1
Breast-feeding and breast cancer in the offspring.后代中的母乳喂养与乳腺癌
Br J Cancer. 1993 Apr;67(4):842-5. doi: 10.1038/bjc.1993.154.
2
Incorporation of viral genome in DNA of chronically infected cells.
Experientia. 1974 May 15;30(5):499-501. doi: 10.1007/BF01926316.
3
Nucleic acid hybridization with RNA immobilized on filter paper.与固定在滤纸上的RNA进行核酸杂交。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2975-8. doi: 10.1073/pnas.69.10.2975.
4
Virus-specific messenger RNA on free and membrane-bound polyribosomes from cells infected with Rauscher leukemia virus.来自感染劳斯氏白血病病毒的细胞的游离及膜结合多核糖体上的病毒特异性信使核糖核酸。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1093-7. doi: 10.1073/pnas.71.4.1093.
5
Synthesis of globin ribonucleic acid from duck-reticulocyte chromatin in vitro.体外从鸭网织红细胞染色质合成珠蛋白核糖核酸。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2029-32. doi: 10.1073/pnas.70.7.2029.
6
Biological and biochemical evidence for an interaction between Marek's disease herpesvirus and avian leukosis virus in vivo.马立克氏病疱疹病毒与禽白血病病毒在体内相互作用的生物学和生物化学证据。
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3175-8. doi: 10.1073/pnas.70.11.3175.
7
Partial transcription of murine type C viral genomes in BALB c cell lines.BALB c细胞系中小鼠C型病毒基因组的部分转录
J Virol. 1973 Oct;12(4):711-20. doi: 10.1128/JVI.12.4.711-720.1973.
8
Virus-specific messenger RNA and nascent polypeptides in polyribosomes of cells replicating murine sarcoma-leukemia viruses.在复制小鼠肉瘤-白血病病毒的细胞的多核糖体中的病毒特异性信使核糖核酸和新生多肽。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2064-8. doi: 10.1073/pnas.70.7.2064.
9
RNA related to that of a murine leukemia virus in Burkitt's tumors and nasopharyngeal carcinomas.与伯基特淋巴瘤和鼻咽癌中鼠白血病病毒相关的RNA。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):5-9. doi: 10.1073/pnas.70.1.5.
10
Detection of RNA-instructed DNA polymerase and high molecular weight RNA in malignant tissue.恶性组织中RNA指导的DNA聚合酶和高分子量RNA的检测
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2020-4. doi: 10.1073/pnas.69.8.2020.
本文引用的文献
1
Sequence complementarity of T2-DNA and T2-specific RNA.T2-DNA与T2特异性RNA的序列互补性。
Proc Natl Acad Sci U S A. 1961 Feb 15;47(2):137-63. doi: 10.1073/pnas.47.2.137.
2
RNA metabolism in the HeLa cell nucleus.海拉细胞核中的RNA代谢
J Mol Biol. 1966 May;17(1):117-30. doi: 10.1016/s0022-2836(66)80098-0.
3
[Acute poisoning in a central hospital. 381 consecutive cases from a 4-year period].[中心医院的急性中毒。4年期间连续收治的381例病例]
Ugeskr Laeger. 1968 May 2;130(18):755-60.
4
Mechanism of viral carcinogenesis by deoxyribonucleic acid mammalian viruses. IV. Related virus-specific ribonucleic acids in tumor cells induced by "highly" oncogenic adenovirus types 12, 18, and 31.脱氧核糖核酸哺乳动物病毒的病毒致癌机制。IV. 由“高”致癌性12型、18型和31型腺病毒诱导的肿瘤细胞中相关病毒特异性核糖核酸
J Virol. 1967 Jun;1(3):576-82. doi: 10.1128/JVI.1.3.576-582.1967.
5
Virus specific RNA in cells transformed by RNA tumour viruses.RNA肿瘤病毒转化细胞中的病毒特异性RNA
Nat New Biol. 1971 Apr 21;230(16):229-32. doi: 10.1038/newbio230229a0.
6
Search for a human breast cancer virus.寻找一种人类乳腺癌病毒。
Nature. 1971 Feb 26;229(5287):611-4. doi: 10.1038/229611a0.
7
RNA-dependent DNA polymerase activity in virus-like particles isolated from human milk.从人乳中分离出的病毒样颗粒中的RNA依赖性DNA聚合酶活性。
Nature. 1971 May 14;231(5298):97-100. doi: 10.1038/231097a0.
8
Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses.致癌RNA病毒中DNA指导的DNA聚合酶产物的特性分析
Nature. 1970 Aug 8;227(5258):563-7. doi: 10.1038/227563a0.
9
SV40-specific RNA in the nucleus and polyribosomes of transformed cells.转化细胞的细胞核和多核糖体中的SV40特异性RNA。
Proc Natl Acad Sci U S A. 1970 Apr;65(4):1089-96. doi: 10.1073/pnas.65.4.1089.
Zotero 插件