Lin Bao-Qin, Zhou Jiu-Yao, Ma Yan, Deng Ying-Jun, Zheng Chuan-Jie, Lin Jun-Li
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China.
Int J Ophthalmol. 2011;4(6):599-604. doi: 10.3980/j.issn.2222-3959.2011.06.05. Epub 2011 Dec 18.
To study the effects of danhong huayu koufuye (DHK) on fasting blood glucose (FBG) and diabetic retinopathy (DR) in streptozotocin (STZ)-induced type 1 diabetic rats to facilitate the rational usage of this drug.
Diabetic rats were induced by injection of a single dose of STZ intraperitoneally at 50mg/kg. Flash electroretinogram (FERG) and oscillatory potentials (OPs) were used to measure retinal function. The microvascular perfusion of ears was performed to study the microcirculation in rats. FBG, body-weight, and 24-h urine volume, water intake and diet intake were also assessed.
DHK had no effect on FBG in normal rats. However, STZ + DHK group were significantly different from those of Model and moved toward those of normal control. It reversed the increase in diet intake (P≤0.05 vs model control) and the loss in body-weight (P≤0.05 vs model control) in diabetic rats. DHK decreased the FBG of diabetic rats by 25.6% (P≤0.05) and 37.9% (P≤0.01) after 14 and 21 days administration as compared with the model control, respectively. Moreover, DHK significantly increased the FERG b-wave amplitude by 80% (P≤0.05 vs model control) and decreased the FERG b-wave latency by 15.3% (P≤0.01 vs model control) after 24 days administration. The OP(1) and OP(2) amplitudes in DHK group were 2.6 (P≤0.01) and 2.0 (P≤0.01) times of model group after 24 days of DHK treatment, respectively. At the same time, OP1 and OP2 latencies in DHK group reduced by 16.0% (P≤0.001) and 14.7% (P≤0.001) as compared with the model control, respectively. Furthermore, the microvascular perfusion of DHK group was 2.4 times of model group (P≤0.001) after 21 days administration.
DHK had no effect on normal FBG. But it had antihyperglycemic activity, and had a preventive and therapeutic effect on DR in diabetic rats.
研究丹红化瘀口服液（DHK）对链脲佐菌素（STZ）诱导的1型糖尿病大鼠空腹血糖（FBG）及糖尿病视网膜病变（DR）的影响，以促进该药物的合理应用。
以50mg/kg的剂量腹腔注射单剂量STZ诱导糖尿病大鼠。采用闪光视网膜电图（FERG）和振荡电位（OPs）测量视网膜功能。进行耳部微血管灌注以研究大鼠的微循环。还评估了FBG、体重、24小时尿量、饮水量和饮食摄入量。
DHK对正常大鼠的FBG无影响。然而，STZ + DHK组与模型组有显著差异，且趋近于正常对照组。它逆转了糖尿病大鼠饮食摄入量的增加（与模型对照组相比，P≤0.05）和体重的减轻（与模型对照组相比，P≤0.05）。与模型对照组相比，给药14天和21天后，DHK分别使糖尿病大鼠的FBG降低了25.6%（P≤0.05）和37.9%（P≤0.01）。此外，给药24天后，DHK使FERG b波振幅显著增加了80%（与模型对照组相比，P≤0.05），并使FERG b波潜伏期缩短了15.3%（与模型对照组相比，P≤0.01）。DHK治疗24天后，DHK组的OP(1)和OP(2)振幅分别是模型组的2.6倍（P≤0.01）和2.0倍（P≤0.01）。同时，与模型对照组相比，DHK组的OP1和OP2潜伏期分别缩短了16.0%（P≤0.001）和14.7%（P≤0.001）。此外，给药21天后，DHK组的微血管灌注是模型组的2.4倍（P≤0.001）。
DHK对正常FBG无影响。但它具有降血糖活性，对糖尿病大鼠的DR有防治作用。
