Institute of Cell and Developmental Biology, Department of Biology, University of Fribourg, Chemin du Musée 10, Fribourg 1700, Switzerland.
Neural Dev. 2012 Jun 20;7:14. doi: 10.1186/1749-8104-7-14.
In Drosophila, most neurogenetic research is carried out in vivo. Mammalian research demonstrates that primary cell culture techniques provide a powerful model to address cell autonomous and non-autonomous processes outside their endogenous environment. We developed a cell culture system in Drosophila using wildtype and genetically manipulated primary neural tissue for long-term observations. We assessed the molecular identity of distinct neural cell types by immunolabeling and genetically expressed fluorescent cell markers. We monitored mitotic activity of cell cultures derived from wildtype and tumorous larval brains. Our system provides a powerful approach to unveil developmental processes in the nervous system and to complement studies in vivo.
在果蝇中，大多数神经遗传学研究是在体内进行的。哺乳动物研究表明，原代细胞培养技术为解决自主和非自主过程提供了一个强大的模型，而无需考虑其内源性环境。我们使用野生型和遗传操作的原代神经组织在果蝇中开发了一种细胞培养系统，用于长期观察。我们通过免疫标记和遗传表达荧光细胞标记物来评估不同神经细胞类型的分子特征。我们监测了源自野生型和肿瘤幼虫大脑的细胞培养的有丝分裂活性。我们的系统提供了一种强大的方法来揭示神经系统的发育过程，并补充体内研究。
