Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.
Plant Physiol Biochem. 2012 Jun;55:77-84. doi: 10.1016/j.plaphy.2012.03.009. Epub 2012 Apr 7.
The effect of phosphate (Pi) starvation on anthocyanin biosynthesis was examined in suspension-cultured grape cells derived from Bailey Alicante A grapes. Cyanindin-3-O-glucoside, peonidin-3-O-glucoside, cyanidin-3-O-(6-O״-p-coumaroyl)-glucoside and peonidin-3-O-(6-O״-p-coumaroyl)-glucoside were detected in cells in both the complete (+Pi)- and Pi-deficient Murashige-Skoog (-Pi) medium but accumulation was much more substantial cells in -Pi cultures. Highest incorporation of [ring-(14)C]phenylalanine into anthocyanins was found at days 3-14 in the cells in -Pi medium. Time-course expression profiles of anthocyanin biosynthetic enzyme genes and a regulatory gene were examined after cells were transferred to the +Pi and -Pi medium. The transcript levels of UFGT and VvmybA1 in -Pi cells were higher than in +Pi cells 24 h after transfer coinciding with the start of anthocyanin accumulation in -Pi cells.
考察了悬浮培养的源自贝利·阿里坎特 A 葡萄的葡萄细胞中磷酸盐（Pi）饥饿对花色苷生物合成的影响。在完整的（+Pi）和 Pi 缺乏的 Murashige-Skoog（-Pi）培养基中的细胞中均检测到矢车菊素-3-O-葡萄糖苷、芍药素-3-O-葡萄糖苷、矢车菊素-3-O-（6-O′-对香豆酰基）-葡萄糖苷和芍药素-3-O-（6-O′-对香豆酰基）-葡萄糖苷，但在-Pi 培养物中的细胞中积累量要大得多。在-Pi 培养基中，细胞中[环-（14）C]苯丙氨酸掺入花色苷的最高值出现在第 3 天至第 14 天。在将细胞转移到+Pi 和-Pi 培养基后，检查了花色苷生物合成酶基因和调节基因的时间进程表达谱。在转移后 24 小时，-Pi 细胞中的 UFGT 和 VvmybA1 的转录水平高于+Pi 细胞，与-Pi 细胞中花色苷积累的开始时间一致。
