Deposition of ultrafine aerosols in rat nasal molds.

To evaluate the health effects of air pollutants on the respiratory tract, it is critical to determine the regional deposition of inhaled aerosols. Information on deposition of larger particles (greater than 0.2 microns) in the nasal passages of laboratory animals is available; the deposition fraction increases with increasing particle size. However, little deposition information is available for ultrafine particles of less than 0.2 microns. Three clear, plastic molds (models) of the nasal passages of F344/N rats, prepared from metal replica casts were used in these studies. Total deposition of ultrafine aerosols in the casts was determined by using a unidirectional flow system. The pressure drops measured in the casts were a function of flow rate to the power of 1.4-1.6, indicating that flow through the nasal passages has nonlaminar components. Deposition data were obtained by using monodisperse sodium chloride aerosols with particle sizes ranging from 0.2 to 0.005 microns, at inspiratory and expiratory flow rates of 200 to 600 ml/min. Similar deposition data were obtained for two of the casts studied. Deposition efficiency was greatest for the smallest particles, and decreased with increasing particle size and flow rate. At an inspiratory flow rate of 400 ml/min, which is comparable to the mean respiratory flow of an adult male F344 rat with a respiratory minute volume of 200 ml, deposition efficiencies reached 40 and 70% for 0.01- and 0.005-microns particles, respectively. These studies demonstrated that turbulent diffusional deposition was the dominant mechanism for uptake of ultrafine particles by the nasal passages.