Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, 500607, India.
J Microbiol Biotechnol. 2012 May;22(5):614-21. doi: 10.4014/jmb.1107.07013.
Silver nanoparticles production by the green chemistry approach was investigated using an isolated marine actinomycetes strain. The isolated strain was identified as Streptomyces albidoflavus based on chemotaxonomic and ribotyping properties. The strain revealed production of silver nanoparticles both extracellular and intracellularly. Surface Plasmon Resonance analysis with the function of time revealed that particle synthesis by this strain is reaction time dependent. The produced particles were spherical shaped and monodispersive in nature and showed a single surface plasmon resonance peak at 410 nm. Size distribution histograms indicated production of 10-40- nm-size nanoparticles with a mean size of 14.5 nm. FT-IR spectra of nanopartilces showed N-H, C-H, and C-N stretching vibrations, denoting the presence of amino acid/ peptide compounds on the surface of silver nanoparticles produced by S. albidoflavus. Synthesized nanoparticles revealed a mean negative zeta potential and electrophoretic mobility of -8.5 mV and -0.000066 cm2/Vs, respectively. The nanoparticles produced were proteinaceous compounds as capping agents with -8.5 mV zeta potential and revealed antimicrobial activity against both Gram-negative and -positive bacterial strains. Owing to their small size, these particles have greater impact on industrial application spectra.
采用绿色化学方法，利用一株分离的海洋放线菌生产了银纳米粒子。根据化学分类和核糖体分型特性，将分离的菌株鉴定为白色链霉菌。该菌株表现出在细胞外和细胞内都能产生银纳米粒子。表面等离子体共振分析的功能随时间变化，表明该菌株的颗粒合成是反应时间依赖的。所产生的粒子呈球形且单分散，在 410nm 处显示单一的表面等离子体共振峰。粒径分布直方图表明，粒径为 10-40nm 的纳米粒子的平均粒径为 14.5nm。纳米粒子的傅里叶变换红外光谱显示 N-H、C-H 和 C-N 伸缩振动，表明白色链霉菌产生的银纳米粒子表面存在氨基酸/肽化合物。合成的纳米粒子的平均负zeta 电位和电泳迁移率分别为-8.5mV 和-0.000066cm2/Vs。所产生的纳米粒子是作为稳定剂的蛋白类化合物，zeta 电位为-8.5mV，对革兰氏阴性和阳性细菌菌株均具有抗菌活性。由于其粒径较小，这些颗粒对工业应用光谱有更大的影响。
