School of Biological Sciences, Universiti Sains Malaysia, Gelugor 11700, Malaysia.
School of Applied Sciences, Faculty of Integrated Life Sciences, Quest International University, Ipoh 30250, Malaysia.
Molecules. 2021 Oct 24;26(21):6414. doi: 10.3390/molecules26216414.
Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs' antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel's properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site.
在这里，我们报告了银纳米粒子（AgNPs）的胞外生物合成及其抗菌和抗癌特性的测定。我们还探索了将生物 AgNPs 掺入纤维素纳米晶体（CNCs）和藻酸盐（Alg）中形成抗菌水凝胶膜的功效。 sp. PBD-311B 被用于 AgNPs 的生物合成。使用紫外-可见光谱、TEM、XRD 和 FTIR 分析对合成的生物 AgNPs 进行了表征。然后，通过 TEMA 和细胞毒性分析测定了生物 AgNPs 的抗菌和抗癌特性。为了形成抗菌水凝胶膜，将生物 AgNPs 与 CNC 和 Alg 溶液混合，并进一步通过 FTIR 分析和圆盘扩散试验进行了表征。合成的生物 AgNPs 的平均尺寸约为 69 ± 2nm，呈球形。XRD 分析证实了银纳米晶的形成。FTIR 分析表明，生物 AgNP 表面存在蛋白质帽，这可能归因于细胞外蛋白质与生物 AgNPs 的结合。生物 AgNPs 对 USM-AR2 和 MRSA 的 MIC 值分别为 6.25mg/mL 和 3.13mg/mL。此外，生物 AgNPs 对癌细胞（DBTRG-0.5MG 和 MCF-7）表现出细胞毒性作用，对正常细胞（SVG-p12 和 MCF-10A）表现出最小的作用，表现出选择性毒性。有趣的是，当生物 AgNPs 掺入 CNC/Alg 中时，它们仍然表现出抑制活性，这意味着水凝胶膜具有抗菌性能。还发现，由于分子间相互作用和水凝胶的性质，生物 AgNP-CNC/Alg 显示出生物 AgNPs 的最小或缓慢释放。总的来说，生物 AgNP-CNC/Alg 是一种有前途的抗菌水凝胶膜，对病原菌和 MRSA 表现出抑制作用，其应用可进一步评估其对癌细胞的抑制作用。它为避免术后伤口感染和手术部位肿瘤复发提供了益处，有利于肿瘤的外科切除。
