LaBS, Department of Structural Engineering, Politecnico di Milano, Milano, Italy.
J Appl Biomater Funct Mater. 2012 Jun 26;10(1):55-65. doi: 10.5301/JABFM.2012.9278.
Three-dimensional material microstructuring by femtosecond laser-induced two-photon polymerization is emerging as an important tool in biomedicine. During two-photon polymerization, a tightly focused femtosecond laser pulse induces a crosslinking photoreaction in the polymer confined within the focal volume. As a rapid-prototyping technique, two-photon polymerization enables the fabrication of truly arbitrary three-dimensional micro- and nano-structures directly from computer models, with a spatial resolution down to 100 nm. In this review, we discuss the fundamentals, experimental methods, and materials used for two-photon polymerization; in addition, we present some applications of this technology related to microfluidics and to biomaterial scaffolds for tissue engineering and regenerative medicine.
飞秒激光诱导的双光子聚合三维材料微结构技术正在成为生物医学领域的重要工具。在双光子聚合过程中，聚焦的飞秒激光脉冲在限定于焦场体积内的聚合物中引发交联光反应。作为一种快速成型技术，双光子聚合能够直接从计算机模型中制造出真正任意的三维微纳结构，其空间分辨率可达 100nm。在本文中，我们讨论了双光子聚合的基本原理、实验方法和所使用的材料；此外，还介绍了该技术在微流控和组织工程及再生医学用生物材料支架方面的一些应用。
