Vilnius University, Faculty of Physics, Department of Quantum Electronics, Laser Research Center, Sauletekio Avenue 10, LT-10223 Vilnius, Lithuania.
J Biomed Opt. 2012 Aug;17(8):081405-1. doi: 10.1117/1.JBO.17.8.081405.
This work presents the latest results on direct laser writing of polymeric materials for tissue engineering applications. A femtosecond Yb:KGW laser (300 fs, 200 kHz, 515 nm) was used as a light source for non-linear lithography. Fabrication was implemented in various photosensitive polymeric materials, such as: hybrid organic-inorganic sol-gel based on silicon-zirconium oxides, commercial ORMOCER® class photoresins. These materials were structured via multi-photon polymerization technique with submicron resolution. Porous three-dimensional scaffolds for artificial tissue engineering were fabricated with constructed system and were up to several millimeters in overall size with 10 to 100 μm internal pores. Biocompatibility of the used materials was tested in primary rabbit muscle-derived stem cell culture in vitro and using laboratory rats in vivo. This interdisciplinary study suggests that proposed technique and materials are suitable for tissue engineering applications.
这项工作展示了用于组织工程应用的直接激光写入聚合物材料的最新研究成果。飞秒 Yb:KGW 激光（300fs、200kHz、515nm）被用作非线性光刻的光源。制造是在各种光敏聚合物材料中实现的，如：基于硅-氧化锆的混合有机-无机溶胶-凝胶、商用 ORMOCER®类光致抗蚀剂。这些材料通过多光子聚合技术以亚微米分辨率进行了结构设计。使用构建系统制造了用于人工组织工程的多孔三维支架，整体尺寸可达数毫米，内部孔径为 10 至 100μm。体外在原代兔肌肉来源干细胞培养中和体内在实验大鼠中测试了所用材料的生物相容性。这项跨学科研究表明，所提出的技术和材料适用于组织工程应用。
