Smith G R, Barton S A, Wallace L M
Institute of Zoology, Zoological Society of London, Regent's Park.
Epidemiol Infect. 1991 Apr;106(2):305-10. doi: 10.1017/s0950268800048457.
It had already been shown with a single virulent strain (A42) of Fusobacterium necrophorum that suspension of the fusobacteria in sub-lethal doses of broth cultures of other bacteria reduced the minimum infective dose (greater than 10(6) organisms) for mice by subcutaneous inoculation, sometimes to less than 10 organisms. The present study extended the known range of bacteria with strong infectivity-enhancing properties to include Bacillus cereus, Klebsiella oxytoca and Staphylococcus aureus; and those with weaker effect to include Bacillus subtilis, 'Bacteroides melaninogenicus', Clostridium sporogenes, Pasteurella haemolytica, and Proteus mirabilis. The study also showed that five further virulent strains of F. necrophorum closely resembled A42 in respect of striking susceptibility to infectivity enhancement by Escherichia coli. Actinomyces (Corynebacterium) pyogenes and S. aureus. One further strain (A6) of F. necrophorum resembled A42 in respect of strong infectivity enhancement by A. pyogenes, S. aureus, B. cereus and K. oxytoca but differed from it and the other five strains in being only slightly affected by E. coli. This work was a necessary prelude to the development of a method, based on infectivity enhancement, for the detection and isolation of F. necrophorum present in small numbers in heavily contaminated material such as faeces.
已经有研究表明，对于坏死梭杆菌的单一强毒株（A42），将其与其他细菌的亚致死剂量肉汤培养物混合悬浮后，通过皮下接种可降低对小鼠的最小感染剂量（大于10⁶个菌），有时可降至小于10个菌。本研究将已知具有强感染增强特性的细菌范围扩大到包括蜡样芽孢杆菌、产酸克雷伯菌和金黄色葡萄球菌；将具有较弱作用的细菌扩大到包括枯草芽孢杆菌、“产黑色素拟杆菌”、产芽孢梭菌、溶血巴斯德菌和奇异变形杆菌。该研究还表明，坏死梭杆菌的另外五个强毒株在对大肠杆菌、化脓放线菌（棒状杆菌属）和金黄色葡萄球菌的感染增强敏感性方面与A42非常相似。坏死梭杆菌的另一个菌株（A6）在化脓放线菌、金黄色葡萄球菌、蜡样芽孢杆菌和产酸克雷伯菌的强感染增强方面与A42相似，但与A42和其他五个菌株不同的是，它仅受大肠杆菌的轻微影响。这项工作是基于感染增强开发一种方法的必要前奏，该方法用于检测和分离存在于粪便等严重污染材料中少量的坏死梭杆菌。
