Smith G R, Till D, Wallace L M, Noakes D E
Nuffield Laboratories of Comparative Medicine, Institute of Zoology, Zoological Society of London, Regent's Park.
Epidemiol Infect. 1989 Jun;102(3):447-58. doi: 10.1017/s0950268800030168.
Necrobacillosis is caused by Fusobacterium necrophorum (FN), but other organisms are often present in the lesions. Their possible role was studied in experiments made with a virulent FN strain which, by itself, produced fatal necrobacillosis in mice provided that large doses (greater than 10(6) organisms, subcutaneously) were given. Mice were inoculated subcutaneously with FN suspended in sub-lethal doses (0.1 ml) of undiluted or diluted broth cultures of other bacteria. Undiluted culture of a strain of Escherichia coli reduced the infective dose of FN to less than 10 organisms; in the necrobacillosis lesions that developed, fusobacteria greatly outnumbered E. coli. A heat-killed preparation or sterile filtrate of E. coli culture had little if any effect on FN. Citrobacter freundii and comparatively small numbers of Corynebacterium (Actinomyces) pyogenes produced effects similar to that of E. coli. An alpha-haemolytic streptococcus, Pseudomonas aeruginosa, Bacteroides fragilis and Fusobacterium nucleatum also enhanced the infectivity of FN, though less strikingly than E. coli. FN increased the persistence in vivo of the alpha-haemolytic streptococcus and B. fragilis, and enabled the latter to multiply profusely.
坏死杆菌病由坏死梭杆菌（FN）引起，但病变中常存在其他微生物。在用一种强毒FN菌株进行的实验中研究了它们可能发挥的作用，该菌株本身若皮下注射大剂量（大于10⁶个菌体）会在小鼠中引发致命的坏死杆菌病。将FN悬浮于其他细菌未稀释或稀释的肉汤培养物的亚致死剂量（0.1 ml）中，给小鼠皮下接种。一株大肠杆菌的未稀释培养物将FN的感染剂量降低至少于10个菌体；在发生的坏死杆菌病病变中，梭杆菌的数量大大超过大肠杆菌。大肠杆菌培养物的热灭活制剂或无菌滤液对FN几乎没有影响。弗氏柠檬酸杆菌和相对少量的化脓棒状杆菌（放线菌）产生了与大肠杆菌类似的效果。一株α溶血性链球菌、铜绿假单胞菌、脆弱拟杆菌和具核梭杆菌也增强了FN的传染性，尽管不如大肠杆菌显著。FN增加了α溶血性链球菌和脆弱拟杆菌在体内的持久性，并使后者大量繁殖。
