Pan Feng, Liu Shuo, Wang Zhe, Shang Peng, Xiao Wen
Key Laboratory of Precision Opto-mechatronics Technology, School of Instrument Science and Optoelectronics Engineering, Beihang University, Beijing, China.
Opt Express. 2012 May 7;20(10):11496-505. doi: 10.1364/OE.20.011496.
The long-term and real-time monitoring the cell division and changes of osteoblasts under simulated zero gravity condition were succeed by combing a digital holographic microscopy (DHM) with a superconducting magnet (SM). The SM could generate different magnetic force fields in a cylindrical cavity, where the gravitational force of biological samples could be canceled at a special gravity position by a high magnetic force. Therefore the specimens were levitated and in a simulated zero gravity environment. The DHM was modified to fit with SM by using single mode optical fibers and a vertically-configured jig designed to hold specimens and integrate optical device in the magnet's bore. The results presented the first-phase images of living cells undergoing dynamic divisions and changes under simulated zero gravity environment for a period of 10 hours. The experiments demonstrated that the SM-compatible DHM setup could provide a highly efficient and versatile method for research on the effects of microgravity on biological samples.
通过将数字全息显微镜（DHM）与超导磁体（SM）相结合，成功实现了在模拟零重力条件下对成骨细胞的细胞分裂和变化进行长期实时监测。超导磁体可在圆柱形腔体内产生不同的磁场力，在特定重力位置，生物样品的重力可被强大的磁力抵消。因此，标本得以悬浮，并处于模拟零重力环境中。通过使用单模光纤和垂直配置的夹具对数字全息显微镜进行了改进，该夹具用于固定标本并将光学装置集成在磁体孔中，以使其与超导磁体相适配。结果呈现了活细胞在模拟零重力环境下进行动态分裂和变化长达10小时的第一阶段图像。实验表明，与超导磁体兼容的数字全息显微镜装置可为研究微重力对生物样品的影响提供一种高效且通用的方法。
