Ecole Polytechnique Federale de Lausanne, Advanced Photonics Laboratory, Lausanne, 1015 Switzerland and Eidgenossische Technische Hochschule Zurich, Space Biology Group, Zurich, 8005 Switzerland.
J Biomed Opt. 2010 Mar-Apr;15(2):026021. doi: 10.1117/1.3377960.
Previous investigations on mammalian cells have shown that microgravity, either that experienced in space, or simulated on earth, causes severe cellular modifications that compromise tissue determination and function. The aim of this study is to investigate, in real time, the morphological changes undergone by cells experiencing simulated microgravity by using digital holographic microscopy (DHM). DHM analysis of living mouse myoblasts (C2C12) is undertaken under simulated microgravity with a random positioning machine. The DHM analysis reveals cytoskeletal alterations similar to those previously reported with conventional methods, and in agreement with conventional brightfield fluorescence microscopy a posteriori investigation. Indeed, DHM is shown to be able to noninvasively and quantitatively detect changes in actin reticular formation, as well as actin distribution, in living unstained samples. Such results were previously only obtainable with the use of labeled probes in conjunction with conventional fluorescence microscopy, with all the classically described limitations in terms of bias, bleaching, and temporal resolution.
先前对哺乳动物细胞的研究表明，无论是在太空中经历的微重力，还是在地球上模拟的微重力，都会导致严重的细胞改变，从而损害组织的确定和功能。本研究的目的是通过数字全息显微镜（DHM）实时研究细胞在模拟微重力下经历的形态变化。使用随机定位机对模拟微重力下的活鼠成肌细胞（C2C12）进行 DHM 分析。DHM 分析显示细胞骨架的改变与以前用常规方法报道的相似，并且与传统明场荧光显微镜的后验研究一致。事实上，DHM 被证明能够非侵入性地定量检测活未染色样品中肌动蛋白网状结构以及肌动蛋白分布的变化。以前，只有使用标记探针结合传统荧光显微镜才能获得这些结果，但在偏倚、漂白和时间分辨率方面存在所有经典描述的限制。
