Division of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium.
J Virol. 2012 Jun;86(11):6369-70. doi: 10.1128/JVI.00641-12.
The complete genome sequence of the giant Pseudomonas phage Lu11 was determined, comparing 454 and Sanger sequencing. The double-stranded DNA (dsDNA) genome is 280,538 bp long and encodes 391 open reading frames (ORFs) and no tRNAs. The closest relative is Ralstonia phage ϕRSL1, encoding 40 similar proteins. As such, Lu11 can be considered phylogenetically unique within the Myoviridae and indicates the diversity of the giant phages within this family.
采用 454 焦磷酸测序和 Sanger 测序法，完成了巨型假单胞菌噬菌体 Lu11 的全基因组序列测定。该双链 DNA（dsDNA）基因组全长 280538bp，编码 391 个开放阅读框（ORFs）和无 tRNA。最接近的亲缘噬菌体是 Ralstonia 噬菌体 ϕRSL1，编码 40 个相似蛋白。因此，Lu11 在肌尾噬菌体科中可被视为系统进化独特的噬菌体，并表明该科巨型噬菌体的多样性。
