Sumi Vikshe, Singh Shambhu Dayal, Dhama Kuldeep, Gowthaman Vasudevan, Barathidasan Rajamani, Sukumar Kuppannan
Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243 122, India.
Trop Anim Health Prod. 2012 Oct;44(7):1791-5. doi: 10.1007/s11250-012-0140-2. Epub 2012 May 10.
In this study, two isolates of infectious bronchitis virus (IBV) from field outbreaks in 2008 (India/LKW/56/IVRI/08) and 2010 (India/NMK/72/IVRI/10) from broiler chickens in India were isolated and characterized. Reverse transcription polymerase chain reaction-restriction fragment length polymorphism of the entire S1 gene revealed that these isolates belong to two different genotypes, India/LKW/56/IVRI/08 as Mass strain whereas India/NMK/72/IVRI/10 as of different genotype. Nucleotide sequencing analysis showed that India/LKW/56/IVRI/08 shared 99 % homology with THA280252 (Thailand) and India/NMK/72/IVRI/10 shared greater than 99 % homology with 4/91 pathogenic strain (UK), JP/Wakayama/2/2004 (Japan) and TA03 (China), while the two Indian IBV isolates shared 73 % identity between them. Phylogenetic data allowed classification of two Indian isolates, India/LKW/56/IVRI/08 as having unique lineage within Mass genotype and India/NMK/72/IVRI/10 as of 4/91 genotype. Our study confirmed the presence of 4/91 (793/B) IBV nephropathogenic strain for the first time in India by virus isolation and sequencing.
在本研究中，从印度肉鸡2008年（印度/LKW/56/IVRI/08）和2010年（印度/NMK/72/IVRI/10）的野外疫情中分离出两株传染性支气管炎病毒（IBV）并进行了鉴定。对整个S1基因进行逆转录聚合酶链反应-限制性片段长度多态性分析表明，这些分离株属于两种不同的基因型，印度/LKW/56/IVRI/08为Mass株，而印度/NMK/72/IVRI/10为不同基因型。核苷酸测序分析表明，印度/LKW/56/IVRI/08与THA280252（泰国）有99%的同源性，印度/NMK/72/IVRI/10与4/91致病株（英国）、JP/和歌山/2/2004（日本）及TA03（中国）有大于99%的同源性，而这两株印度IBV分离株之间的同一性为73%。系统发育数据使两株印度分离株得以分类，印度/LKW/56/IVRI/08在Mass基因型内具有独特的谱系，印度/NMK/72/IVRI/10属于4/91基因型。我们的研究通过病毒分离和测序首次在印度证实了4/91（793/B）IBV肾致病性毒株的存在。
