• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长靶序列杂交测序。

Sequencing by hybridization of long targets.

机构信息

School of Engineering and Applied Sciences and Kavli Institute for Bionano Science and Technology, Harvard University, Cambridge, Massachusetts, United States of America.

出版信息

PLoS One. 2012;7(5):e35819. doi: 10.1371/journal.pone.0035819. Epub 2012 May 4.

DOI:10.1371/journal.pone.0035819
PMID:22574124
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3344849/
Abstract

Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.

摘要

杂交测序 (SBH) 从其生化确定的 l 长子序列重建 n 长目标 DNA 序列。在标准方法中，由于重复子序列导致重建退化，因此可以唯一重建的均匀随机序列的长度限制为 n = O(2(l))。我们提出了一种改进的测序方法，该方法克服了这一限制，而无需不同类型的生化测定，并且对错误具有鲁棒性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/2b2699fc6e64/pone.0035819.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/792fcba898a4/pone.0035819.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/6d7614d503a4/pone.0035819.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/ba68537d5b64/pone.0035819.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/c38920b5c2df/pone.0035819.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/c714b9208f09/pone.0035819.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/2b2699fc6e64/pone.0035819.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/792fcba898a4/pone.0035819.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/6d7614d503a4/pone.0035819.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/ba68537d5b64/pone.0035819.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/c38920b5c2df/pone.0035819.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/c714b9208f09/pone.0035819.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dacb/3344849/2b2699fc6e64/pone.0035819.g006.jpg

相似文献

1
Sequencing by hybridization of long targets.长靶序列杂交测序。
PLoS One. 2012;7(5):e35819. doi: 10.1371/journal.pone.0035819. Epub 2012 May 4.
2
Dealing with repetitions in sequencing by hybridization.处理杂交测序中的重复问题。
Comput Biol Chem. 2006 Oct;30(5):313-20. doi: 10.1016/j.compbiolchem.2006.05.002. Epub 2006 Aug 30.
3
Sequencing-by-hybridization revisited: the analog-spectrum proposal.重新审视杂交测序：模拟频谱方案。
IEEE/ACM Trans Comput Biol Bioinform. 2004 Jan-Mar;1(1):46-52. doi: 10.1109/TCBB.2004.12.
4
Sequencing of pools of nucleic acids on oligonucleotide arrays.在寡核苷酸阵列上对核酸池进行测序。
Biosystems. 1993;30(1-3):215-31. doi: 10.1016/0303-2647(93)90072-k.
5
Multistage sequencing by hybridization.多阶段杂交测序法。
J Comput Biol. 1998 Spring;5(1):165-71. doi: 10.1089/cmb.1998.5.165.
6
Poisson process approximation for sequence repeats, and sequencing by hybridization.序列重复的泊松过程近似法及杂交测序法。
J Comput Biol. 1996 Fall;3(3):425-63. doi: 10.1089/cmb.1996.3.425.
7
Sequencing by hybridization in the presence of hybridization errors.存在杂交错误时的杂交测序法。
Genome Inform Ser Workshop Genome Inform. 2000;11:53-62.
8
Detection of short repeated genomic sequences on metaphase chromosomes using padlock probes and target primed rolling circle DNA synthesis.使用锁式探针和靶标引发滚环DNA合成技术检测中期染色体上的短重复基因组序列。
BMC Mol Biol. 2007 Nov 13;8:103. doi: 10.1186/1471-2199-8-103.
9
Rearrangements of DNA sequences and SBH.DNA序列重排与SBH
Comput Chem. 1994 Sep;18(3):221-3. doi: 10.1016/0097-8485(94)85016-x.
10
Multiplex sequencing by hybridization.基于杂交的多重测序
J Comput Biol. 2001;8(2):141-9. doi: 10.1089/106652701300312913.

引用本文的文献

1
Next-Generation Sequencing and Triple-Negative Breast Cancer: Insights and Applications.下一代测序技术与三阴性乳腺癌：相关研究进展与临床应用
Int J Mol Sci. 2023 Jun 2;24(11):9688. doi: 10.3390/ijms24119688.
2
Overview of Next-Generation Sequencing Technologies.新一代测序技术概述
Curr Protoc Mol Biol. 2018 Apr;122(1):e59. doi: 10.1002/cpmb.59.
3
An Algorithm for Sequencing by Hybridization Based on an Alternating DNA Chip.基于交替 DNA 芯片的杂交测序算法。

本文引用的文献

1
High-throughput injection with microfluidics using picoinjectors.采用皮升注射器的微流控高通量注射。
Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19163-6. doi: 10.1073/pnas.1006888107. Epub 2010 Oct 20.
2
Probabilistic nucleotide assembling method for sequencing by hybridization.用于杂交测序的概率核苷酸组装方法。
Bioinformatics. 2004 Sep 22;20(14):2181-8. doi: 10.1093/bioinformatics/bth202. Epub 2004 Apr 8.
3
High-throughput variation detection and genotyping using microarrays.使用微阵列进行高通量变异检测和基因分型。
Interdiscip Sci. 2018 Sep;10(3):605-615. doi: 10.1007/s12539-017-0220-0. Epub 2017 Feb 28.
Genome Res. 2001 Nov;11(11):1913-25. doi: 10.1101/gr.197201.
4
Optimal reconstruction of a sequence from its probes.根据其探测值对序列进行最优重建。
J Comput Biol. 1999 Fall-Winter;6(3-4):361-8. doi: 10.1089/106652799318328.
5
Poisson process approximation for sequence repeats, and sequencing by hybridization.序列重复的泊松过程近似法及杂交测序法。
J Comput Biol. 1996 Fall;3(3):425-63. doi: 10.1089/cmb.1996.3.425.
6
The probability of unique solutions of sequencing by hybridization.杂交测序独特解决方案的概率。
J Comput Biol. 1994 Summer;1(2):105-10. doi: 10.1089/cmb.1994.1.105.
7
DNA sequence determination by hybridization: a strategy for efficient large-scale sequencing.通过杂交进行DNA序列测定：一种高效大规模测序的策略。
Science. 1993 Jun 11;260(5114):1649-52. doi: 10.1126/science.8503011.
8
A general method applicable to the search for similarities in the amino acid sequence of two proteins.一种适用于寻找两种蛋白质氨基酸序列相似性的通用方法。
J Mol Biol. 1970 Mar;48(3):443-53. doi: 10.1016/0022-2836(70)90057-4.
9
A novel method for nucleic acid sequence determination.一种用于核酸序列测定的新方法。
J Theor Biol. 1988 Dec 7;135(3):303-7. doi: 10.1016/s0022-5193(88)80246-7.
10
Sequencing of megabase plus DNA by hybridization: theory of the method.通过杂交对百万碱基以上DNA进行测序：该方法的理论
Genomics. 1989 Feb;4(2):114-28. doi: 10.1016/0888-7543(89)90290-5.