Bains W, Smith G C
Department of Biochemistry, University of Bath, U.K.
J Theor Biol. 1988 Dec 7;135(3):303-7. doi: 10.1016/s0022-5193(88)80246-7.
We describe a novel sequencing methodology which should be readily and completely automated. The method relies on fragmentation of a nucleotide or deoxynucleotide sequence into short fragments, and subsequent quantitation of the fragments by hybridization to oligo-deoxynucleotides on a solid support. The original sequence may be reconstructed from the resulting table of fragment frequencies. We present a specific protocol which would allow practical implementation of this approach.
我们描述了一种新颖的测序方法，该方法应易于完全自动化。该方法依赖于将核苷酸或脱氧核苷酸序列片段化为短片段，随后通过与固体支持物上的寡脱氧核苷酸杂交来对片段进行定量。原始序列可以从所得的片段频率表中重建。我们提出了一种具体方案，该方案将允许这种方法的实际应用。
