Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
Infect Genet Evol. 2012 Oct;12(7):1413-8. doi: 10.1016/j.meegid.2012.04.027. Epub 2012 May 3.
Dengue virus infection causes significant morbidity and mortality in humans world-wide. The Aedes aegypti mosquito is the major vector that spreads dengue virus to humans. Interaction between dengue viruses and A. aegypti is a multi-factorial phenomena that is determined by both virus and mosquito genotypes. Although, studies have suggested significant association of mosquito vectorial capacity with population variation of dengue virus, specifications of the vector factors that may influence vector-virus compatibility are very limited in the literature. Recently, we have shown that a large number of genes are differentially expressed between MOYO-S (susceptible) and MOYO-R (refractory) A. aegypti strains upon infection with dengue virus (JAM-1409 genotype). In the current study, we show that specific intrinsic features of A. aegypti genes are significantly associated with 'responsiveness' of mosquito genes to dengue infection. Binomial logistic regression analysis further reveals differential marginal effects of these features on gene responsiveness of mosquitoes to the viral infection. Thus, our result shows that intrinsic features of genes significantly affect differential expression of A. aegypti genes to dengue infection. The information will benefit further investigations on evolution of genes among natural populations of A. aegypti conferring differential susceptibility to dengue virus.
登革热病毒感染在全球范围内给人类造成了重大的发病率和死亡率。埃及伊蚊是将登革热病毒传播给人类的主要媒介。登革热病毒与埃及伊蚊之间的相互作用是一种多因素现象，由病毒和蚊子基因型共同决定。尽管有研究表明，蚊子的媒介能力与登革热病毒的人群变异之间存在显著关联，但文献中对可能影响媒介-病毒相容性的媒介因素的具体规定非常有限。最近，我们已经表明，在感染登革热病毒（JAM-1409 基因型）后，大量基因在易感（MOYO-S）和抗性（MOYO-R）埃及伊蚊品系之间表现出差异表达。在本研究中，我们表明，埃及伊蚊基因的特定内在特征与蚊子基因对登革热感染的“反应性”显著相关。二项逻辑回归分析进一步揭示了这些特征对蚊子基因对病毒感染的反应性的差异边际效应。因此，我们的结果表明，基因的内在特征显著影响了埃及伊蚊基因对登革热感染的差异表达。这些信息将有助于进一步研究埃及伊蚊自然种群中基因的进化，这些基因赋予了对登革热病毒的不同敏感性。
