Molecular Biology Unit, National Centre for Cell Science, Ganeshkhind, Pune, Maharashtra, India.
Parasitol Res. 2013 Jul;112(7):2627-37. doi: 10.1007/s00436-013-3428-x. Epub 2013 May 1.
Differences in midgut bacterial communities of Aedes aegypti, the primary mosquito vector of dengue viruses (DENV), might influence the susceptibility of these mosquitoes to infection by DENV. As a first step toward addressing this hypothesis, comparative analysis of bacterial communities from midguts of mosquito strains with differential genetic susceptibility to DENV was performed. 16S rRNA gene libraries and real-time PCR approaches were used to characterize midgut bacterial community composition and abundance in three Aedes aegypti strains: MOYO, MOYO-R, and MOYO-S. Although Pseudomonas spp.-related clones were predominant across all libraries, some interesting and potentially significant differences were found in midgut bacterial communities among the three strains. Pedobacter sp.- and Janthinobacterium sp.-related phylotypes were identified only in the MOYO-R strain libraries, while Bacillus sp. was detected only in the MOYO-S strain. Rahnella sp. was found in MOYO-R and MOYO strains libraries but was absent in MOYO-S libraries. Both 16S rRNA gene library and real-time PCR approaches confirmed the presence of Pedobacter sp. only in the MOYO-R strain. Further, real-time PCR-based quantification of 16S rRNA gene copies showed bacterial abundance in midguts of the MOYO-R strain mosquitoes to be at least 10-100-folds higher than in the MOYO-S and MOYO strain mosquitoes. Our study identified some putative bacteria with characteristic physiological properties that could affect the infectivity of dengue virus. This analysis represents the first report of comparisons of midgut bacterial communities with respect to refractoriness and susceptibility of Aedes aegypti mosquitoes to DENV and will guide future efforts to address the potential interactive role of midgut bacteria of Aedes aegypti mosquitoes in determining vectorial capacity for DENV.
登革病毒（DENV）的主要传播媒介埃及伊蚊（Aedes aegypti）的中肠细菌群落的差异可能影响这些蚊子对 DENV 感染的易感性。为了验证这一假说，首先对具有不同遗传易感性的 DENV 的埃及伊蚊菌株的中肠细菌群落进行了比较分析。使用 16S rRNA 基因文库和实时 PCR 方法来描述三种埃及伊蚊品系（MOYO、MOYO-R 和 MOYO-S）的中肠细菌群落组成和丰度。尽管在所有文库中都以假单胞菌相关克隆为主，但在这三种品系的中肠细菌群落中发现了一些有趣且潜在重要的差异。仅在 MOYO-R 菌株文库中鉴定到 Pedobacter sp. 和 Janthinobacterium sp. 相关的类群，而仅在 MOYO-S 菌株中检测到芽孢杆菌。在 MOYO-R 和 MOYO 菌株文库中发现了 Rahnella sp.，但在 MOYO-S 文库中不存在。16S rRNA 基因文库和实时 PCR 方法均证实仅在 MOYO-R 菌株中存在 Pedobacter sp.。此外，基于实时 PCR 的 16S rRNA 基因拷贝定量显示，MOYO-R 菌株蚊子中肠细菌的丰度至少比 MOYO-S 和 MOYO 菌株蚊子高 10-100 倍。我们的研究确定了一些具有可能影响登革热病毒感染力的特征生理特性的假定细菌。这一分析代表了首次对埃及伊蚊的中肠细菌群落进行比较，以了解其对 DENV 的抵抗力和易感性，并将指导未来努力确定埃及伊蚊中肠细菌在决定 DENV 媒介能力方面的潜在交互作用。
