使用单细胞微腔阵列芯片对大量群体中的单个细胞进行变形测量。
Deformation measurement of individual cells in large populations using a single-cell microchamber array chip.
作者信息
Doh I, Lee W C, Cho Y-H, Pisano A P, Kuypers F A
出版信息
Appl Phys Lett. 2012 Apr 23;100(17):173702-1737023. doi: 10.1063/1.4704923.
Abstract
We analyze the deformability of individual red blood cells (RBCs) using SiCMA technology. Our approach is adequate to quickly measure large numbers of individual cells in heterogeneous populations. Individual cells are trapped in a large-scale array of micro-wells, and dielectrophoretic (DEP) force is applied to deform the cells. The simple structures of micro-wells and DEP electrodes facilitate the analysis of thousands of RBCs in parallel. This unique method allows the correlation of red cell deformation with cell surface and cytosolic characteristics to define the distribution of individual cellular characteristics in heterogeneous populations.
摘要
我们使用碳化硅微阵列技术分析单个红细胞（RBC）的可变形性。我们的方法足以快速测量异质群体中的大量单个细胞。单个细胞被捕获在大规模的微孔阵列中，并施加介电泳（DEP）力使细胞变形。微孔和DEP电极的简单结构便于并行分析数千个红细胞。这种独特的方法能够将红细胞变形与细胞表面及胞质特征相关联，以确定异质群体中单个细胞特征的分布。
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