Department of Operative Dentistry, School of Dentistry, University of Granada, Granada, Spain.
J Endod. 2012 Jun;38(6):826-8. doi: 10.1016/j.joen.2012.01.013. Epub 2012 Feb 24.
The use of chelating agents in endodontic treatment is required to remove the smear layer and to achieve better disinfection within the root canal system. The aim of this study was to evaluate the residual antimicrobial activity of 17% ethylenediaminetetraacetic acid (EDTA), 7% maleic acid (MA), and 10% lactic acid (LA) alone and combined with 0.2% cetrimide (CTR) on infected root canals with Enterococcus faecalis.
Biofilms of E. faecalis were grown in uniradicular roots for 4 weeks. After preparation the roots were randomly divided into 6 groups (n = 15) according to the final irrigation regimen as follows: 5 mL of 17% EDTA; 5 mL of 17% EDTA + 0.2% CTR; 5 mL of 7% MA; 5 mL of 7% MA + 0.2% CTR; 5 mL of 10% LA; and 5 mL of 10% LA + 0.2% CTR. Samples were collected for 60 days to denote the presence of bacterial growth. Fisher and Pearson χ(2) tests were applied to compare the percentages of specimens without E. faecalis regrowth at all the time periods.
There were significant differences in bacterial regrowth between the groups with and without CTR. There were not significant differences over time among the 3 groups with CTR or among the 3 without CTR.
The residual activity against E. faecalis of 7% MA + 0.2% CTR, 17% EDTA + 0.2% CTR, and 10% LA + 0.2% CTR was greater than the use of chelating agents alone.
在根管治疗中使用螯合剂是为了去除玷污层，并在根管系统内实现更好的消毒。本研究的目的是评估 17%乙二胺四乙酸（EDTA）、7%马来酸（MA）和 10%乳酸（LA）单独使用以及与 0.2%十六烷基三甲基溴化铵（CTR）联合使用对感染的粪肠球菌根管的残留抗菌活性。
粪肠球菌生物膜在单根牙中培养 4 周。准备后，根据最终冲洗方案将根随机分为 6 组（n = 15），如下所示：5 mL 17% EDTA；5 mL 17% EDTA + 0.2% CTR；5 mL 7% MA；5 mL 7% MA + 0.2% CTR；5 mL 10% LA；和 5 mL 10% LA + 0.2% CTR。收集样本 60 天，以表示是否存在细菌生长。应用 Fisher 和 Pearson χ(2)检验比较所有时间段无粪肠球菌再生长标本的百分比。
有和无 CTR 的组之间细菌再生长存在显著差异。有 CTR 的 3 组之间或无 CTR 的 3 组之间随时间无显著差异。
7% MA + 0.2% CTR、17% EDTA + 0.2% CTR 和 10% LA + 0.2% CTR 对粪肠球菌的残留活性大于单独使用螯合剂。
