King Saud University, College of Pharmacy, Dept. of Pharmacognosy, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Phytochemistry. 2012 Jul;79:129-40. doi: 10.1016/j.phytochem.2012.04.003. Epub 2012 May 16.
Caralluma sinaica is sold on local markets of Saudi Arabia for various health benefits however no phytochemical study has specifically been performed on this species. NMR and UHPLC-ESI-TOF-MS profilings of the ethanolic extract of the whole plant reveal a very complex phytochemical composition dominated by pregnanes. Detailed information on its constituents was obtained after isolation. Six pregnane glycosides were obtained and characterized based on the extensive spectroscopic analysis (including IR, ¹H NMR, ¹³C NMR and MS data), in addition to ten known compounds (seven pregnanes and three flavonoids). The compounds were identified as 12β-O-benzoyl-20-O-acetyl boucerin-3-O-6-deoxy-3-O-methyl-β-D-glucopyranosyl-(1-->4)-β-D-cymaropyranosyl-(1-->4)-β-D-cymaropyranoside, 12β-O-tigloyl-20-O-acetyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-β-D-cymaropyranoside, 12β-O-benzoyl-20-O-acetyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-β-D-digitalopyranosyl-(1-->4)-β-D-cymaropyranosyl-(1-->4)-β-D-cymaropyranoside, 12β-O-benzoyl-20-O-acetyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-hevetopyranosyl-(1-->4)-β-D-cymaropyranosyl-(1-->4)-β-D-cymaropyranoside, 12β-O-benzoyl-20-O-tigloyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-β-D-cymaropyranoside, 12β-20-O-dibenzoyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-β-D-cymaropyranosyl-(1-->4)-β-D-cymaropyranoside. Finally, the isolated compounds were evaluated for their quinone reductase induction.
沙葫芦在沙特阿拉伯的当地市场上销售，具有多种健康益处，但尚未针对该物种进行专门的植物化学研究。对整株植物的乙醇提取物进行 NMR 和 UHPLC-ESI-TOF-MS 分析，揭示了一种非常复杂的植物化学成分，主要由孕烷组成。通过分离获得了详细的成分信息。从分离得到的六种孕烷糖苷中，基于广泛的光谱分析（包括 IR、1H NMR、13C NMR 和 MS 数据），并结合 10 种已知化合物（7 种孕烷和 3 种类黄酮）进行了鉴定。这些化合物被鉴定为 12β-O-苯甲酰基-20-O-乙酰基布塞林-3-O-6-去氧-3-O-甲基-β-D-吡喃葡萄糖基-(1-->4)-β-D-刺槐糖苷、12β-O- tigloyl-20-O-acetyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-β-D-cymaropyranoside、12β-O-benzoyl-20-O-acetyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-β-D-digitalopyranosyl-(1-->4)-β-D-cymaropyranosyl-(1-->4)-β-D-cymaropyranoside、12β-O-benzoyl-20-O-acetyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-hevetopyranosyl-(1-->4)-β-D-cymaropyranosyl-(1-->4)-β-D-cymaropyranoside、12β-O-benzoyl-20-O-tigloyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-β-D-cymaropyranoside、12β-O-benzoyl-20-O-tigloyl boucerin-3-O-β-D-glucopyranosyl-(1-->4)-β-D-cymaropyranoside。最后，对分离得到的化合物进行了醌还原酶诱导活性评价。
