Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Phytochemistry. 2013 Apr;88:54-60. doi: 10.1016/j.phytochem.2012.12.005. Epub 2013 Jan 9.
In a previous study, the methanolic extract as well as the chloroform fraction of the aerial parts of Caralluma quadrangula (Forssk.) N.E.Br. indigenous to Saudi Arabia showed significant in vitro cytotoxic activity against breast cancer (MCF7) cell line. In a biologically-guided fractionation approach, four acylated pregnane glycosides were isolated from the chloroform fraction of C. quadrangula. The structures of the isolated compounds were elucidated by the analysis of their MS and NMR data. The compounds were identified as 12,20-di-O-benzoylboucerin 3-O-β-D-digitoxopyranosyl-(1→4)-β-D-canaropyranosyl-(1→4)-β-D-cymaropyranoside (1), 12,20-di-O-benzoylboucerin 3-O-β-D-cymaropyranosyl-(1→4)-β-D-canaropyranosyl-(1→4)-β-D-cymaropyranoside (2), 12,20-di-O-benzoylboucerin 3-O-β-D-glucopyranosyl-(1→4)-β-D-digitoxopyranosyl-(1→4)-β-D-canaropyranosyl-(1→4)-β-D-cymaropyranoside (3) and 12,20-di-O-benzoyl-3β,5α,12β,14β,20-pentahydroxy-(20R)-pregn-6-ene 3-O-β-D-glucopyranosyl-(1→4)-β-D-digitoxopyranosyl-(1→4)-β-D-canaropyranosyl-(1→4)-β-D-cymaropyranoside (4). The isolated compounds were tested for their cytotoxic activity against breast cancer (MCF7) cell line.
在之前的一项研究中，产自沙特阿拉伯的 Caralluma quadrangula（Forssk.）N.E.Br. 的甲醇提取物和氯仿部分对乳腺癌（MCF7）细胞系表现出显著的体外细胞毒性活性。在生物导向的分段方法中，从 C. quadrangula 的氯仿部分分离出四种酰基孕烷糖苷。通过分析它们的 MS 和 NMR 数据阐明了分离化合物的结构。这些化合物被鉴定为 12,20-二-O-苯甲酰基boucerin 3-O-β-D-地芰糖基-(1→4)-β-D-加拿大麻糖基-(1→4)-β-D-毛地黄糖苷（1），12,20-二-O-苯甲酰基boucerin 3-O-β-D-毛地黄糖苷基-(1→4)-β-D-加拿大麻糖基-(1→4)-β-D-毛地黄糖苷（2），12,20-二-O-苯甲酰基boucerin 3-O-β-D-吡喃葡萄糖基-(1→4)-β-D-地芰糖基-(1→4)-β-D-加拿大麻糖基-(1→4)-β-D-毛地黄糖苷（3）和 12,20-二-O-苯甲酰基-3β,5α,12β,14β,20-五羟基-(20R)-孕甾-6-烯 3-O-β-D-吡喃葡萄糖基-(1→4)-β-D-地芰糖基-(1→4)-β-D-加拿大麻糖基-(1→4)-β-D-毛地黄糖苷（4）。对分离出的化合物进行了对乳腺癌（MCF7）细胞系的细胞毒性活性测试。
