Labbe R G, Rey D K
Appl Environ Microbiol. 1979 Jun;37(6):1196-200. doi: 10.1128/aem.37.6.1196-1200.1979.
Replacement of starch with raffinose in Duncan and Strong sporulation medium improved percent sporulation in six of eight strains tested. Enterotoxin concentration in cell extracts was increased in the case of four of five known enterotoxin-positive strains. With strain NCTC 10240, levels of 0.3, 0.4, and 0.5% raffinose produced the highest enterotoxin concentration 300 to 320 micrograms of enterotoxin per mg of cell extract protein. At a level of 0.4% raffinose the highest enterotoxin concentration in cell extracts of NCTC 10240 occurred after 8 h of growth in Duncan and Strong medium. Enterotoxin produced in the presence of starch or raffinose by three separate strains all migrated at similar Rm by polyacrylamide gel electrophoresis.
在邓肯和斯特朗芽孢形成培养基中用棉子糖替代淀粉，在所测试的8个菌株中有6个的芽孢形成率得到了提高。在5个已知产肠毒素阳性菌株中，有4个菌株的细胞提取物中的肠毒素浓度有所增加。对于菌株NCTC 10240，0.3%、0.4%和0.5%的棉子糖水平产生了最高的肠毒素浓度，即每毫克细胞提取物蛋白中含有300至320微克肠毒素。在0.4%棉子糖水平下，NCTC 10240细胞提取物中的最高肠毒素浓度出现在邓肯和斯特朗培养基中生长8小时后。由3个不同菌株在淀粉或棉子糖存在下产生的肠毒素，通过聚丙烯酰胺凝胶电泳在相似的迁移率（Rm）下迁移。
